AB68136

Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal p130 phospho S952 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications.

View Alternative Names

RB2, RBL2, Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab68136 (unpurified) at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Unknown

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

All lanes:

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/500000 dilution

Lane 1:

Jurkat cell lysate, untreated at 10 µg

Lane 2:

Jurkat cell lysate, treated with AP at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-mouse at 1/2000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Lab

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

All lanes:

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Lab

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

All lanes:

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/2000 dilution

Lane 1:

Mouse heart lysates. Then the membrane was incubated with phosphatase at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Lab

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

All lanes:

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (ab68136) at 1/5000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates. Then the membrane was incubated with phosphatase at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 128 kDa

Observed band size: 128 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2387AY

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/10000 - 1/500000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "For unpurified use at 1/10000 - 1/500000.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rb2 p130 also known as Retinoblastoma-like protein 2 functions as an important regulator of cell cycle progression. It has a molecular weight of about 130 kDa and members of the retinoblastoma (Rb) protein family which also includes p107 and RB1. These proteins help control the transition from the G1 to S phase during the cell cycle. Rb2 p130 is widely expressed in various tissues particularly in quiescent cells where it plays a role in maintaining cells in the G0 phase.

Biological function summary

The protein acts as a fundamental component of the DREAM (DP RB-like E2F and MuvB) complex which plays an important role in repressing the transcription of E2F target genes. This repression ensures the proper regulation of cell proliferation and differentiation. The protein contributes to maintaining genomic stability by helping to control the entry of cells into the DNA synthesis phase thereby acting as a tumor suppressor.

Pathways

Rb2 p130 is heavily involved in the cell cycle control and proliferation pathways. It associates with the E2F family of transcription factors which are critical in transcription regulation of genes important for cell cycle progression. It modulates activities within the p53 pathway as well another pathway important for cell cycle arrest and apoptosis. This relation highlights its interplay with proteins like cyclin-dependent kinases (CDKs) which regulate cell cycle transitions through phosphorylation events.

The protein has been linked to several cancer types due to its role as a tumor suppressor. Disruptions in its expression or function can lead to unchecked cellular division paving the way for tumorigenesis. Mutations or alterations in the Rb pathway involving Rb2 p130 RB1 and p107 can contribute to the development of cancers such as retinoblastoma and lung carcinoma. The involvement of these proteins signifies their importance in maintaining controlled cell division with impairments correlating strongly to cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

See full target information RBL2 pS952

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1615300 PubMed40703674

2025

inhibits porcine epidemic diarrhea virus infection by disrupting nucleocapsid protein-p53 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Hongde Lu,Haoyang Liu,Ning Guo,Yu Zhou,Haiyan Lu,Zhiyuan He,Hong Dong

Journal of the American Heart Association 11:e024764 PubMed36129061

2022

Loss of Rbl2 (Retinoblastoma-Like 2) Exacerbates Myocardial Ischemia/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jingrui Chen,Peng Xia,Yuening Liu,Clark Kogan,Zhaokang Cheng

Journal of virology 95:e0018721 PubMed34037422

2021

Coronavirus Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Interacts with p53 To Induce Cell Cycle Arrest in S-Phase and Promotes Viral Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjun Su,Da Shi,Xiaoxu Xing,Shanshan Qi,Dan Yang,Jiyu Zhang,Yuru Han,Qinghe Zhu,Haibo Sun,Xiaoran Wang,Haoyang Wu,Meijiao Wang,Shan Wei,Chunqiu Li,Donghua Guo,Li Feng,Dongbo Sun

Leukemia 30:86-93 PubMed26174628

2015

Dual inhibition of CDK4/Rb and PI3K/AKT/mTOR pathways by ON123300 induces synthetic lethality in mantle cell lymphomas.

Applications

Unspecified application

Species

Unspecified reactive species

S K A Divakar,M V Ramana Reddy,S C Cosenza,S J Baker,D Perumal,A C Antonelli,J Brody,B Akula,S Parekh,E Premkumar Reddy

Nucleic acids research 43:6860-73 PubMed26071952

2015

p27Kip1 and p21Cip1 collaborate in the regulation of transcription by recruiting cyclin-Cdk complexes on the promoters of target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Orlando,Edurne Gallastegui,Arnaud Besson,Gabriel Abril,Rosa Aligué,Maria Jesus Pujol,Oriol Bachs

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rb2 p130 (phospho S952) antibody [EP2387AY]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Western blot - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] (AB68136)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise