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Rabbit Recombinant Monoclonal p130 phospho S952 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Not recommended
Expected
Tested
Mouse
Not recommended
Expected
Predicted

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

1 products for Alternative Version

Target data

Function

Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Alternative names

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Rabbit Recombinant Monoclonal p130 phospho S952 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP2387AY

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab243908 is the carrier-free version of Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Activity summary

Rb2 p130 also known as Retinoblastoma-like protein 2 functions as an important regulator of cell cycle progression. It has a molecular weight of about 130 kDa and members of the retinoblastoma (Rb) protein family which also includes p107 and RB1. These proteins help control the transition from the G1 to S phase during the cell cycle. Rb2 p130 is widely expressed in various tissues particularly in quiescent cells where it plays a role in maintaining cells in the G0 phase.

Biological function summary

The protein acts as a fundamental component of the DREAM (DP RB-like E2F and MuvB) complex which plays an important role in repressing the transcription of E2F target genes. This repression ensures the proper regulation of cell proliferation and differentiation. The protein contributes to maintaining genomic stability by helping to control the entry of cells into the DNA synthesis phase thereby acting as a tumor suppressor.

Pathways

Rb2 p130 is heavily involved in the cell cycle control and proliferation pathways. It associates with the E2F family of transcription factors which are critical in transcription regulation of genes important for cell cycle progression. It modulates activities within the p53 pathway as well another pathway important for cell cycle arrest and apoptosis. This relation highlights its interplay with proteins like cyclin-dependent kinases (CDKs) which regulate cell cycle transitions through phosphorylation events.

Associated diseases and disorders

The protein has been linked to several cancer types due to its role as a tumor suppressor. Disruptions in its expression or function can lead to unchecked cellular division paving the way for tumorigenesis. Mutations or alterations in the Rb pathway involving Rb2 p130 RB1 and p107 can contribute to the development of cancers such as retinoblastoma and lung carcinoma. The involvement of these proteins signifies their importance in maintaining controlled cell division with impairments correlating strongly to cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 (unpurified) at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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