Rabbit Recombinant Monoclonal p130 phospho S952 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111903.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (AB243908)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Expected | Tested |
| Mouse | Not recommended | Expected | Predicted |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 products for Alternative Version
Target data
Function
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
Alternative names
Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2, RBL2, RB2
Recommended products
Rabbit Recombinant Monoclonal p130 phospho S952 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.
Alternative names
Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2, p130, RBR-2, RBL2, RB2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP2387AY
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab243908 is the carrier-free version of Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Rb2 p130 also known as Retinoblastoma-like protein 2 functions as an important regulator of cell cycle progression. It has a molecular weight of about 130 kDa and members of the retinoblastoma (Rb) protein family which also includes p107 and RB1. These proteins help control the transition from the G1 to S phase during the cell cycle. Rb2 p130 is widely expressed in various tissues particularly in quiescent cells where it plays a role in maintaining cells in the G0 phase.
- Biological function summary
The protein acts as a fundamental component of the DREAM (DP RB-like E2F and MuvB) complex which plays an important role in repressing the transcription of E2F target genes. This repression ensures the proper regulation of cell proliferation and differentiation. The protein contributes to maintaining genomic stability by helping to control the entry of cells into the DNA synthesis phase thereby acting as a tumor suppressor.
- Pathways
Rb2 p130 is heavily involved in the cell cycle control and proliferation pathways. It associates with the E2F family of transcription factors which are critical in transcription regulation of genes important for cell cycle progression. It modulates activities within the p53 pathway as well another pathway important for cell cycle arrest and apoptosis. This relation highlights its interplay with proteins like cyclin-dependent kinases (CDKs) which regulate cell cycle transitions through phosphorylation events.
- Associated diseases and disorders
The protein has been linked to several cancer types due to its role as a tumor suppressor. Disruptions in its expression or function can lead to unchecked cellular division paving the way for tumorigenesis. Mutations or alterations in the Rb pathway involving Rb2 p130 RB1 and p107 can contribute to the development of cancers such as retinoblastoma and lung carcinoma. The involvement of these proteins signifies their importance in maintaining controlled cell division with impairments correlating strongly to cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111903.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer with alkaline phosphatase treatment (image A) and treated without alkaline phosphatase (image B) tissue sections labeling Rb2 p130 with purified Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 at 1/600 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908), expandable thumbnail](https://content.abcam.com/products/images/rb2-p130-phospho-s952-antibody-ep2387ay-bsa-and-azide-free-ab243908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] - BSA and Azide free (ab243908)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136 (unpurified) at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Rb2 p130 (phospho S952) antibody [EP2387AY] ab68136).
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