Rabbit Polyclonal RBBP4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Dog samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human RBBP4 aa 400 to C-terminus.
Images
Publications
Filter by
- Journal of cell science 136:2023The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoshan L Shrestha,Vinutha Balachandra,Jee Hun Kim,Austin Rossi,Pranathi Vadlamani,Subhash Chandra Sethi,Laurent Ozbun,Shinjen Lin,Ken Chin-Chien Cheng,Raj Chari,Tatiana S Karpova,Gianluca Pegoraro,Daniel R Foltz,Natasha J Caplen,Munira A BasraiPubMed 37129573
- The CRISPR journal 5:769-7862022A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClarence Mills,Andrew Riching,Ashleigh Keller,Jesse Stombaugh,Amanda Haupt,Elena Maksimova,Sarah M Dickerson,Emily Anderson,Kevin Hemphill,Chris Ebmeier,John A Schiel,Josien Levenga,Matthew Perkett,Anja van Brabant Smith,Zaklina StrezoskaPubMed 36257604
- Cancers 13:2021HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza CerdánPubMed 34572914
- Journal of neuroinflammation 18:1972021LncRNA HOXA-AS2 regulates microglial polarization via recruitment of PRC2 and epigenetic modification of PGC-1α expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodong Yang,Yi Zhang,Yimeng Chen,Xiaoqin He,Yiwei Qian,Shaoqing Xu,Chao Gao,Chengjun Mo,Shengdi Chen,Qin XiaoPubMed 34511122
- Cell chemical biology 26:1573-1585.e102019Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMay Bakail et. al.PubMed 31543461
- PLoS pathogens 14:e10073132018Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamille Cohen et. al.PubMed 30235352
- Nature communications 9:28292018Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li et. al.PubMed 30026490
- Molecular medicine reports 18:1571-15812018Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxi Jin et. al.PubMed 29901205
- EMBO reports 18:1801-18162017LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingya Sun et. al.PubMed 28808113
- BMC molecular biology 17:242016Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes.Applications:WBReactive species:Unspecified reactive speciesNicholas W Ashton et. al.PubMed 27938330
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RBBP4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q09028
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Expected | Predicted | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Dog | Expected | Predicted | Expected | Expected |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Mouse, Dog | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
Alternative names
RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4
Recommended products
Rabbit Polyclonal RBBP4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Dog samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human RBBP4 aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RBBP4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q09028
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Locus link: 350005
Alternative names: similar to chromatin assembly factor 1 p48 subunit
A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RBBP4 antibody (ab1765)
RBBP4 Western blot staining of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using rabbit Anti-RBBP4 antibody
Lane 1: Western blot - Anti-RBBP4 antibody (ab1765) at 1/500 dilution
Lane 2: Western blot - Anti-RBBP4 antibody (ab1765) at 1/1000 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/2000 dilution
Predicted band size: 48 kDa
Western blot - Anti-RBBP4 antibody (ab1765)
All lanes: Western blot - Anti-RBBP4 antibody (ab1765) at 1/1000 dilution
All lanes: Human LCL whole cell lysate
SecondaryAll lanes: Anti-rabbit IgG HRP
Developed using the ECL technique.
Predicted band size: 48 kDa
Observed band size: 40 kDa, 55 kDa
Exposure time: 1min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com