AB1765

Anti-RBBP4 antibody

Name: Anti-RBBP4 antibody (ab1765) | Abcam
Brand: Abcam Limited
SKU: AB1765
Rating: 5 (8 reviews)

(8 Reviews)

(19 Publications)

Rabbit Polyclonal RBBP4 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human, Dog samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human RBBP4 aa 400 to C-terminus.

View Alternative Names

RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4

2 Images

AbReview3853****

Western blot - Anti-RBBP4 antibody (AB1765)

All lanes:

Western blot - Anti-RBBP4 antibody (ab1765) at 1/1000 dilution

All lanes:

Human LCL whole cell lysate

Secondary

All lanes:

Anti-rabbit IgG HRP

Predicted band size: 48 kDa

Observed band size: 40 kDa,55 kDa

true

Exposure time: 1min

Unknown

Western blot - Anti-RBBP4 antibody (AB1765)

Lane 1:

Western blot - Anti-RBBP4 antibody (ab1765) at 1/500 dilution

Lane 2:

Western blot - Anti-RBBP4 antibody (ab1765) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/2000 dilution

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Dog

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human RBBP4 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q09028

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Locus link: 350005 Alternative names: similar to chromatin assembly factor 1 p48 subunit

A protein complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; Histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. P48 can bind to histone H4 in the absence of CAF-1 P150 and P160. Binds directly to helix 1 of histone H4, a region that is not accessible when H4 is in chromatin. SUBUNIT: CAF-1 is composed of three subunits, P48, P60 AND P150. Only minor amounts of P48 are complexed with P60 AND P150 in G1 phase. P48/RBBP4 is also part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodelling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SUV39H1 and HDAC7 (By similarity).

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA (PubMed : 10866654). Component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair (PubMed : 8858152). Component of the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression (PubMed : 9150135). Component of the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling (PubMed : 16428440, PubMed : 28977666, PubMed : 39460621). Component of the PRC2 complex, which promotes repression of homeotic genes during development (PubMed : 29499137, PubMed : 31959557). Component of the NURF (nucleosome remodeling factor) complex (PubMed : 14609955, PubMed : 15310751).

See full target information RBBP4

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37129573

2023

The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability.

Applications

Unspecified application

Species

Unspecified reactive species

Roshan L Shrestha,Vinutha Balachandra,Jee Hun Kim,Austin Rossi,Pranathi Vadlamani,Subhash Chandra Sethi,Laurent Ozbun,Shinjen Lin,Ken Chin-Chien Cheng,Raj Chari,Tatiana S Karpova,Gianluca Pegoraro,Daniel R Foltz,Natasha J Caplen,Munira A Basrai

The CRISPR journal 5:769-786 PubMed36257604

2022

A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Clarence Mills,Andrew Riching,Ashleigh Keller,Jesse Stombaugh,Amanda Haupt,Elena Maksimova,Sarah M Dickerson,Emily Anderson,Kevin Hemphill,Chris Ebmeier,John A Schiel,Josien Levenga,Matthew Perkett,Anja van Brabant Smith,Zaklina Strezoska

Cancers 13: PubMed34572914

2021

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza Cerdán

Journal of neuroinflammation 18:197 PubMed34511122

2021

LncRNA HOXA-AS2 regulates microglial polarization via recruitment of PRC2 and epigenetic modification of PGC-1α expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Yang,Yi Zhang,Yimeng Chen,Xiaoqin He,Yiwei Qian,Shaoqing Xu,Chao Gao,Chengjun Mo,Shengdi Chen,Qin Xiao

Nature communications 11:24 PubMed31911655

2020

The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Min Jang,Jenny F Nathans,Haiqing Fu,Christophe E Redon,Lisa M Jenkins,Bhushan L Thakur,Lőrinc S Pongor,Adrian M Baris,Jacob M Gross,Maura J OʹNeill,Fred E Indig,Steven D Cappell,Mirit I Aladjem

Cell chemical biology 26:1573-1585.e10 PubMed31543461

2019

Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1.

Applications

Unspecified application

Species

Unspecified reactive species

May Bakail,Albane Gaubert,Jessica Andreani,Gwenaëlle Moal,Guillaume Pinna,Ekaterina Boyarchuk,Marie-Cécile Gaillard,Regis Courbeyrette,Carl Mann,Jean-Yves Thuret,Bérengère Guichard,Brice Murciano,Nicolas Richet,Adeline Poitou,Claire Frederic,Marie-Hélène Le Du,Morgane Agez,Caroline Roelants,Zachary A Gurard-Levin,Geneviève Almouzni,Nadia Cherradi,Raphael Guerois,Françoise Ochsenbein

PLoS pathogens 14:e1007313 PubMed30235352

2018

Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Cohen,Armelle Corpet,Simon Roubille,Mohamed Ali Maroui,Nolwenn Poccardi,Antoine Rousseau,Constance Kleijwegt,Olivier Binda,Pascale Texier,Nancy Sawtell,Marc Labetoulle,Patrick Lomonte

Nature communications 9:2829 PubMed30026490

2018

Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Huajie Song,Hong Mei,Erhu Fang,Xiaojing Wang,Feng Yang,Huanhuan Li,Yajun Chen,Kai Huang,Liduan Zheng,Qiangsong Tong

Molecular medicine reports 18:1571-1581 PubMed29901205

2018

Overexpression of retinoblastoma‑binding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3‑kinase/protein kinase B pathway suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxi Jin,Rui Jiang,Yongsheng Xiang,Zhen Fan,Zhiwei Wu,Bo Yang,Lujun Yang,Shanshan Wei,Yan Yang

EMBO reports 18:1801-1816 PubMed28808113

2017

LncRNA GAS5 inhibits microglial M2 polarization and exacerbates demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Dingya Sun,Zhongwang Yu,Xue Fang,Mingdong Liu,Yingyan Pu,Qi Shao,Dan Wang,Xiaolin Zhao,Aijun Huang,Zhenghua Xiang,Chao Zhao,Robin Jm Franklin,Li Cao,Cheng He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RBBP4 antibody

Western blot - Anti-RBBP4 antibody (AB1765)

Western blot - Anti-RBBP4 antibody (AB1765)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

Product promise