Anti-RBBP4 antibody [EPR3412] - ChIP Grade

6 product images

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  ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 μg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
  Additional screenshots of mapped reads can be downloaded here.

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  Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  All lanes: Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution

  Lane 1: HeLa lysate at 10 µg

  Lane 2: Jurkat cell lysate at 10 µg

  Lane 3: Raji cell lysate at 10 µg

  Lane 4: 293T cell lysate at 10 µg

  Secondary

  All lanes: goat anti-rabbit HRP at 1/2000 dilution

  Predicted band size: 48 kDa

  Observed band size: 48 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
  

  Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

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  ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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  ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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  ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

  ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.