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Rabbit Recombinant Monoclonal RBBP4 antibody. Suitable for IHC-P, WB, ChIP-seq, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 3 publications.


Images

ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344), expandable thumbnail
  • Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBChIP-seqChIC/CUT&RUN-seq
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/20000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
8 µg for 107 Cells
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RBBP4 antibody. Suitable for IHC-P, WB, ChIP-seq, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3412
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 μg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
    Additional screenshots of mapped reads can be downloaded here.

  • Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    All lanes: Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution

    Lane 1: HeLa lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: Raji cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com