Anti-RBBP4 antibody [EPR3412] - ChIP Grade

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

ab92344 at 1/250 dilution staining RBBP4 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 μg of ab92344 [EPR3412]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. Additional screenshots of mapped reads can be downloaded here.

• WB

Unknown

Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

All lanes:

Western blot - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (ab92344) at 1/20000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-RBBP4 antibody [EPR3412] - ChIP Grade (AB92344)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab92344 [EPR3412]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.