AB154755

Anti-RbBP5 antibody

Name: Anti-RbBP5 antibody (ab154755) | Abcam
Brand: Abcam Limited
SKU: AB154755

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(4 Publications)

Rabbit Polyclonal RBBP5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBBP5 aa 1-250.

View Alternative Names

RBQ3, RBBP5, Retinoblastoma-binding protein 5, RBBP-5, Retinoblastoma-binding protein RBQ-3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (AB154755)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (AB154755)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling RbBP5 with ab154755 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

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Western blot - Anti-RbBP5 antibody (AB154755)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RbBP5 antibody (ab154755) at 1/500 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBBP5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q15291

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RbBP5 also known as Retinoblastoma binding protein 5 acts as a core component of the Set1/COMPASS complex which plays a role in histone methylation. The RbBP5 protein weighs approximately 58 kDa and it is found throughout various tissues with notable expression in areas engaged in active transcription. This widespread presence suggests its substantial impact on chromatin modification processes.

Biological function summary

RbBP5 functions to assist in the regulation of gene transcription. It is an integral part of the Set1/COMPASS complex which is responsible for the tri-methylation of histone H3 at lysine 4 (H3K4me3) a modification associated with transcriptionally active genes. By participating in this complex RbBP5 contributes to the establishment and maintenance of euchromatin regions thereby allowing transcription factors and other proteins involved in gene expression to access DNA more easily.

Pathways

RbBP5 plays a role in chromatin remodeling and transcription regulation pathways. Its involvement in the H3K4me3 modification process places it in the TRX pathway influencing various gene expression patterns. RbBP5 interacts with other components of the Set1/COMPASS complex including ASH2L and WDR5 which collaboratively modulate the levels of histone methylation and ultimately affect gene transcription activities.

RbBP5 has connections to cancer and developmental disorders due to its role in gene expression regulation. Abnormal RbBP5 activity can disrupt proper H3K4 methylation which may lead to either the inappropriate activation or silencing of genes implicating it in oncogenic processes. In cancer progression interactions between RbBP5 and other components like the MLL protein (mixed-lineage leukemia) become significant as mutations or dysregulation within this complex contribute to the pathogenesis of leukemia and other malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). Does not affect ES cell self-renewal (By similarity). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 19556245). In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

See full target information RBBP5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:829 PubMed40442479

2025

RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Guangqi Yan,Hao Li,Chunyu Wang,Ye Kang,Shengli Wang,Wei Liu,Lin Lin,Renlong Zou,Kai Zeng,Manlin Wang,Ruina Luan,Baosheng Zhou,Yu Bai,Dongjun Yang,Bolin Ning,Ge Sun,Yue Zhao

Journal of experimental & clinical cancer research : CR 41:77 PubMed35209949

2022

Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Baoyu He,Hongli Pan,Fengque Zheng,Saiqiong Chen,Qingli Bie,Jinghe Cao,Rou Zhao,Jing Liang,Li Wei,Jianchao Zeng,Hui Li,Xing Cui,Yixuan Ding,Wei Chao,Tiantian Xiang,Yuhe Cheng,Gui Qiu,Shishun Huang,Libo Tang,Jiansheng Chang,Delan Luo,Jie Yang,Bin Zhang

Liver international : official journal of the International Association for the Study of the Liver 40:240-251 PubMed31612616

2019

miR-4510 blocks hepatocellular carcinoma development through RAF1 targeting and RAS/RAF/MEK/ERK signalling inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Amani Ghousein,Nicola Mosca,Flora Cartier,Justine Charpentier,Jean-William Dupuy,Anne-Aurélie Raymond,Paulette Bioulac-Sage,Christophe F Grosset

Cell stem cell 23:396-411.e8 PubMed30146411

2018

Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Hernandez,Zheng Wang,Bulat Ramazanov,Yin Tang,Sameet Mehta,Cheryl Dambrot,Yu-Wei Lee,Kaleab Tessema,Ishan Kumar,Michael Astudillo,Thomas A Neubert,Shangqin Guo,Natalia B Ivanova

View all publications

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Product promise

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Anti-RbBP5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (AB154755)

Western blot - Anti-RbBP5 antibody (AB154755)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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