Rabbit Polyclonal RBBP5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBBP5 aa 1-250.
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Publications
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- Journal of experimental & clinical cancer research : CR 41:772022Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaoyu He,Hongli Pan,Fengque Zheng,Saiqiong Chen,Qingli Bie,Jinghe Cao,Rou Zhao,Jing Liang,Li Wei,Jianchao Zeng,Hui Li,Xing Cui,Yixuan Ding,Wei Chao,Tiantian Xiang,Yuhe Cheng,Gui Qiu,Shishun Huang,Libo Tang,Jiansheng Chang,Delan Luo,Jie Yang,Bin ZhangPubMed 35209949
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RBBP5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q15291
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Target data
Function
In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). Does not affect ES cell self-renewal (By similarity). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed:19131338). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3 (PubMed:19556245). Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:19556245). In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Alternative names
RBQ3, RBBP5, Retinoblastoma-binding protein 5, RBBP-5, Retinoblastoma-binding protein RBQ-3
Recommended products
Rabbit Polyclonal RBBP5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBBP5 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RBBP5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q15291
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RbBP5 also known as Retinoblastoma binding protein 5 acts as a core component of the Set1/COMPASS complex which plays a role in histone methylation. The RbBP5 protein weighs approximately 58 kDa and it is found throughout various tissues with notable expression in areas engaged in active transcription. This widespread presence suggests its substantial impact on chromatin modification processes.
- Biological function summary
RbBP5 functions to assist in the regulation of gene transcription. It is an integral part of the Set1/COMPASS complex which is responsible for the tri-methylation of histone H3 at lysine 4 (H3K4me3) a modification associated with transcriptionally active genes. By participating in this complex RbBP5 contributes to the establishment and maintenance of euchromatin regions thereby allowing transcription factors and other proteins involved in gene expression to access DNA more easily.
- Pathways
RbBP5 plays a role in chromatin remodeling and transcription regulation pathways. Its involvement in the H3K4me3 modification process places it in the TRX pathway influencing various gene expression patterns. RbBP5 interacts with other components of the Set1/COMPASS complex including ASH2L and WDR5 which collaboratively modulate the levels of histone methylation and ultimately affect gene transcription activities.
- Associated diseases and disorders
RbBP5 has connections to cancer and developmental disorders due to its role in gene expression regulation. Abnormal RbBP5 activity can disrupt proper H3K4 methylation which may lead to either the inappropriate activation or silencing of genes implicating it in oncogenic processes. In cancer progression interactions between RbBP5 and other components like the MLL protein (mixed-lineage leukemia) become significant as mutations or dysregulation within this complex contribute to the pathogenesis of leukemia and other malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-RbBP5 antibody (ab154755)
7.5% SDS PAGE
All lanes: Western blot - Anti-RbBP5 antibody (ab154755) at 1/500 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 59 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (ab154755)
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling RbBP5 with ab154755 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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