Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

RbBP5 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab322656 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322656 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab322656 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322656 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-RbBP5 antibody [EPR27128-112] (<a href='/en-us/products/primary-antibodies/rbbp5-antibody-epr27128-112-ab322656'>ab322656</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322656 [EPR27128-112]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322656 [EPR27128-112]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Supplier Data

ChIP-sequencing - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab322656 [EPR27128-112]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• WB

Supplier Data

Western blot - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 15 seconds; Lane 3 : 59 seconds.

All lanes:

Western blot - Anti-RbBP5 antibody [EPR27128-112] (<a href='/en-us/products/primary-antibodies/rbbp5-antibody-epr27128-112-ab322656'>ab322656</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

• WB

Supplier Data

Western blot - Anti-RbBP5 antibody [EPR27128-112] - BSA and Azide free (AB324822)

This data was developed using ab322656, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band at approximately 40 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 103 seconds; Lane 3 : 26 seconds.

All lanes:

Western blot - Anti-RbBP5 antibody [EPR27128-112] (<a href='/en-us/products/primary-antibodies/rbbp5-antibody-epr27128-112-ab322656'>ab322656</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting RbBP5 whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,36 kDa

false