Anti-RBFOX3 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 antibody (AB325715)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing cells stained by ab325715. The sections were stained with anti-NeuN polyclonal antibody (visualized in green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 antibody (AB325715)

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus region showing cells stained by ab325715. The sections were stained with anti-NeuN polyclonal antibody (visualized in green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei.

• WB

Supplier Data

Western blot - Anti-RBFOX3 antibody (AB325715)

4-12% Bis-Tris Gel was loaded with 10ug/lane of rat brain nucleus-enriched fraction. Proteins were transferred to PVDF membrane, blocked with 5% non-fat milk powder, and then washed with PBS containing 0.1% Tween. Primary antibody was incubated with the membrane at a concentration of 1 : 5000 and then detected via chemiluminescence (30sec) using a Goat Anti-Chicken H+L, HRP conjugated secondary antibody. The antibody recognizes endogenous RBFOX3 in the rat brain nucleus-enriched fraction as a doublet at the expected molecular weights of around 46 and 48 kDa.

All lanes:

Western blot - Anti-RBFOX3 antibody (ab325715) at 1/5000 dilution

All lanes:

rat brain nucleus-enriched fraction at 10 µg

Secondary

All lanes:

Goat Anti-Chicken H+L, HRP conjugated secondary antibody

Observed band size: 46 kDa,48 kDa

false

Exposure time: 30s