AB327220

Anti-RBFOX3 Antibody

Name: Anti-RBFOX3 Antibody (ab327220) | Abcam
Brand: Abcam Limited
SKU: AB327220
Price: 560 USD
Availability: InStock

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Rabbit Polyclonal NeuN antibody. Suitable for IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NeuN.

View Alternative Names

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemical staining of human cerebellum using ab327220 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in cells in granular layer.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemical staining of human cerebral cortex using ab327220 at 1 : 200–1 : 500 dilution, showing strong nuclear and cytoplasmic positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemical staining of human hippocampus using ab327220 at 1 : 200–1 : 500 dilution, showing strong nuclear and cytoplasmic positivity in pyramidal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using ab327220 at 1 : 200–1 : 500 dilution. Corresponding RBFOX3 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence - Anti-RBFOX3 Antibody (AB327220)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RBFOX3 Antibody (AB327220)

Immunofluorescent staining of human cell line A549 using ab327220 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP

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ChIP - Anti-RBFOX3 Antibody (AB327220)

ChIP-Exo-Seq composite graph for ab327220 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemical staining of human tonsil using ab327220 at 1 : 200–1 : 500 dilution, showing no positivity in germinal center cells as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBFOX3 Antibody (AB327220)

Immunohistochemical staining of human skeletal muscle using ab327220 at 1 : 200–1 : 500 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NeuN.

A6NFN3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

See full target information NeuN

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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