Rabbit Polyclonal RBM10/S1-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.
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Publications
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- Genes 13:2022A TARP Syndrome Phenotype Is Associated with a Novel Splicing Variant in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Owczarek-Lipska,Fenja Markus,Eva Bültmann,G Christoph Korenke,John NeidhardtPubMed 36421828
- Nature chemical biology 18:643-6512022Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiongwen Cao,Alexandra Khitun,Cecelia M Harold,Carson J Bryant,Shu-Jian Zheng,Susan J Baserga,Sarah A SlavoffPubMed 35393574
- Oncology letters 23:1542022NPTX1 inhibits pancreatic cancer cell proliferation and migration and enhances chemotherapy sensitivity by targeting RBM10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wu,Gaifang Liu,Kang An,Linping ShiPubMed 35836482
- Nucleic acids research 48:6824-68382020Dengue virus targets RBM10 deregulating host cell splicing and innate immune response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBerta Pozzi,Laureano Bragado,Pablo Mammi,María Florencia Torti,Nicolás Gaioli,Leopoldo G Gebhard,Martín E García Solá,Rita Vaz-Drago,Néstor G Iglesias,Cybele C García,Andrea V Gamarnik,Anabella SrebrowPubMed 32432721
- International journal of oncology 54:467-4782018Functional role of RBM10 in lung adenocarcinoma proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuna Sun,Mengqi Jia,Wei Sun,Lu Feng,Chundong Gu,Taihua WuPubMed 30483773
- Molecular cell 66:270-284.e132017A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Treiber,Nora Treiber,Uwe Plessmann,Simone Harlander,Julia-Lisa Daiß,Norbert Eichner,Gerhard Lehmann,Kevin Schall,Henning Urlaub,Gunter MeisterPubMed 28431233
- RNA biology 14:45-572016The RNA-binding landscape of RBM10 and its role in alternative splicing regulation in models of mouse early development.Applications:WB, CLIPReactive species:MouseJulie Rodor,David R FitzPatrick,Eduardo Eyras,Javier F CáceresPubMed 27763814
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A) (By similarity). May bind to specific miRNA hairpins (PubMed:28431233).
Alternative names
RNA-binding protein 10, G patch domain-containing protein 9, RNA-binding motif protein 10, RNA-binding protein S1-1, S1-1, RBM10, DXS8237E, GPATC9, GPATCH9, KIAA0122
Recommended products
Rabbit Polyclonal RBM10/S1-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab72423 was affinity purified using an epitope specific to RBM10/S1-1 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-RBM10/S1-1 antibody (ab72423)
All lanes: Western blot - Anti-RBM10/S1-1 antibody (ab72423) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 104 kDa
Observed band size: 120 kDa
Exposure time: 3min
Immunoprecipitation - Anti-RBM10/S1-1 antibody (ab72423)
Detection of RBM10/S1-1 by Western Blot of Immunprecipitate.
ab72423 at 1μg/ml staining RBM10/S1-1 in HeLa whole cell lysate immunoprecipitated using ab72423 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-RBM10/S1-1 antibody (ab72423)
Predicted band size: 104 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody (ab72423)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling RBM10 with ab72423 at 1/200 (1µg/ml). Detection: DAB.
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