AB224149

Anti-RBM10/S1-1 antibody - N-terminal

Name: Anti-RBM10/S1-1 antibody - N-terminal (ab224149) | Abcam
Brand: Abcam Limited
SKU: AB224149
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RBM10/S1-1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM10 aa 1-100.

View Alternative Names

DXS8237E, GPATC9, GPATCH9, KIAA0122, RBM10, RNA-binding protein 10, G patch domain-containing protein 9, RNA-binding motif protein 10, RNA-binding protein S1-1, S1-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

Paraffin-embedded human small intestine tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

Shows moderate to strong nuclear positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RBM10/S1-1 (green) using ab224149 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

Paraffin-embedded human skeletal muscle tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

Paraffin-embedded human cerebellum tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

Paraffin-embedded human prostate tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RBM10/S1-1 antibody - N-terminal (AB224149)

All lanes:

Western blot - Anti-RBM10/S1-1 antibody - N-terminal (ab224149) at 1/100 dilution

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 104 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RBM10 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P98175

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200.00000 - 1/500.00000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04000-0.40000 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2.00000 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A) (By similarity). May bind to specific miRNA hairpins (PubMed : 28431233).

See full target information RBM10

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 14:969-980 PubMed35283645

2022

Integrated Bioinformatics Analysis and Validation of the Prognostic Value of Expression in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Jie Pang,Zhe Sun,Wen-Feng Lu,Hui Si-Ma,Zhi-Peng Lin,Yang Shi,Ying-Cheng Yang,Xi-Jun Zhao,Guang-Shun Yang,Guang-Zhi Jin,Ning Yang

Cancer discovery 11:1736-1753 PubMed33707235

2021

Genetic Determinants of EGFR-Driven Lung Cancer Growth and Therapeutic Response .

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Foggetti,Chuan Li,Hongchen Cai,Jessica A Hellyer,Wen-Yang Lin,Deborah Ayeni,Katherine Hastings,Jungmin Choi,Anna Wurtz,Laura Andrejka,Dylan G Maghini,Nicholas Rashleigh,Stellar Levy,Robert Homer,Scott N Gettinger,Maximilian Diehn,Heather A Wakelee,Dmitri A Petrov,Monte M Winslow,Katerina Politi

Cell proliferation 53:e12823 PubMed32515533

2020

LncRNA BBOX1-AS1 upregulates HOXC6 expression through miR-361-3p and HuR to drive cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Xu,Baohua Yang,Lifeng Wang,Yunheng Zhu,Xiuxiang Zhu,Ziyin Xia,Zhen Zhao,Ling Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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