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Rabbit Polyclonal RBM10/S1-1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM10 aa 1-100.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human RBM10 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P98175
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

May be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A) (By similarity). May bind to specific miRNA hairpins (PubMed:28431233).

Alternative names

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Rabbit Polyclonal RBM10/S1-1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM10 aa 1-100.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human RBM10 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P98175
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Western blot - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    All lanes: Western blot - Anti-RBM10/S1-1 antibody - N-terminal (AB224149) at 1/100 dilution

    All lanes: A549 (human lung carcinoma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 104 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    Paraffin-embedded human cerebellum tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    Paraffin-embedded human prostate tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    Paraffin-embedded human small intestine tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

    Shows moderate to strong nuclear positivity in glandular cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    Paraffin-embedded human skeletal muscle tissue stained for RBM10/S1-1 using ab224149 at 1/200 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-RBM10/S1-1 antibody - N-terminal (ab224149), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RBM10/S1-1 antibody - N-terminal (ab224149)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for RBM10/S1-1 (green) using ab224149 at 4 μg/ml in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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