Rabbit Polyclonal RBM14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 13 publications.
Images
Publications
Filter by
- World journal of surgical oncology 21:1322023RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Hu,Hanshuo Mu,Zhiping DengPubMed 37060064
- BMC genomics 24:1462023Study of FOXO1-interacting proteins using TurboID-based proximity labeling technology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanting Su,Yuanyuan Guo,Jieyu Guo,Ting Zeng,Ting Wang,Wu LiuPubMed 36964488
- Blood cancer discovery 4:180-2072023In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Vujovic,Laura de Rooij,Ava Keyvani Chahi,He Tian Chen,Brian A Yee,Sampath K Loganathan,Lina Liu,Derek C H Chan,Amanda Tajik,Emily Tsao,Steven Moreira,Pratik Joshi,Joshua Xu,Nicholas Wong,Zaldy Balde,Soheil Jahangiri,Sasan Zandi,Stefan Aigner,John E Dick,Mark D Minden,Daniel Schramek,Gene W Yeo,Kristin J HopePubMed 36763002
- Frontiers in molecular biosciences 9:10051362022Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHikari Narabayashi,Chiharu Koma,Kazuaki Nakata,Mion Ikegami,Yusuke Nakanishi,Jun Ogihara,Masato Tsuda,Akira Hosono,Shigemasa Hanazawa,Kyoko TakahashiPubMed 36339704
- Frontiers in cell and developmental biology 9:6357282021RBM14 Modulates Tubulin Acetylation and Regulates Spindle Morphology During Meiotic Maturation in Mouse Oocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Qin,Yi Qu,Yi-Feng Yuan,Yang-Yang Li,Jie QiaoPubMed 33604343
- Proceedings of the National Academy of Sciences of 117:5329-53382020Intrinsically disordered protein RBM14 plays a role in generation of RNA:DNA hybrids at double-strand break sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumi Jang et. al.PubMed 32094185
- mSphere 3:2018Nucleolar Relocalization of RBM14 by Influenza A Virus NS1 Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrant Beyleveld,Daniel J Chin,Elena Moreno Del Olmo,Jade Carter,Isabel Najera,Cristian Cillóniz,Megan L ShawPubMed 30429226
- Virology 522:193-1982018HIV-1 replication in CD4 T cells exploits the down-regulation of antiviral NEAT1 long non-coding RNAs following T cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongbing Liu,Pei-Wen Hu,Jacob Couturier,Dorothy E Lewis,Andrew P RicePubMed 30036787
- Cell cycle (Georgetown, Tex.) 16:1175-11802017RNA-binding protein RBM14 regulates dissociation and association of non-homologous end joining proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas E Simon et. al.PubMed 28426349
- eLife 5:2016Circadian RNA expression elicited by 3'-UTR IRAlu-paraspeckle associated elements.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Ferret | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1 (PubMed:11443112). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (PubMed:25385835). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728). Also involved in the regulation of pre-mRNA alternative splicing (PubMed:37548402).
Alternative names
SIP, RBM14, RNA-binding protein 14, Paraspeckle protein 2, RNA-binding motif protein 14, RRM-containing coactivator activator/modulator, Synaptotagmin-interacting protein, PSP2, SYT-interacting protein
Recommended products
Rabbit Polyclonal RBM14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunoprecipitation - Anti-RBM14 antibody (ab70636)
Detection of RBM14 by Western Blot of Immunprecipitate.
ab70636 at 1µg/ml staining RBM14 in HeLa whole cell lysate immunoprecipitated using ab70636 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-RBM14 antibody (ab70636)
Predicted band size: 69 kDa
Western blot - Anti-RBM14 antibody (ab70636)
All lanes: Western blot - Anti-RBM14 antibody (ab70636) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 69 kDa
Exposure time: 30s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com