Rabbit Polyclonal Rbm15/OTT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM15 aa 250-400.
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- Environmental toxicology 38:950-9612023RBM15 silencing promotes ferroptosis by regulating the TGF-β/Smad2 pathway in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Feng,Yaling Li,Fen He,Fuwei ZhangPubMed 36715115
- Cell proliferation 56:e133442022Circ-CTNNB1 drives aerobic glycolysis and osteosarcoma progression via m6A modification through interacting with RBM15.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Yang,Yangyang Liu,Jun Xiao,Bo Li,Yajun Chen,Anpei Hu,Jin Zeng,Zhili Liu,Hucheng LiuPubMed 36181462
- Journal of experimental & clinical cancer research : CR 40:802021RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Wang,Linli Tian,Yushan Li,Jingting Wang,Bingrui Yan,Like Yang,Qiuying Li,Rui Zhao,Ming Liu,Peng Wang,Yanan SunPubMed 33637103
- OncoTargets and therapy 13:10785-107952020To Develop and Validate the Combination of RNA Methylation Regulators for the Prognosis of Patients with Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Zhang et. al.PubMed 33122917
- Frontiers in endocrinology 10:4152019Cyclophosphamide Regulates N6-Methyladenosine and m6A RNA Enzyme Levels in Human Granulosa Cells and in Ovaries of a Premature Ovarian Aging Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoxian Huang et. al.PubMed 31316467
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RBM15 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T37
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (By similarity). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed:27602518). Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (By similarity). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation (PubMed:26575292). Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen (By similarity). Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL (By similarity). May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing (PubMed:17001072, PubMed:19786495). High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing (PubMed:17001072, PubMed:19786495). May be implicated in HOX gene regulation (PubMed:11344311).
Alternative names
OTT, OTT1, RBM15, RNA-binding protein 15, One-twenty two protein 1, RNA-binding motif protein 15
Recommended products
Rabbit Polyclonal Rbm15/OTT antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBM15 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RBM15 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q96T37
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rbm15 also known as OTT is a multifunctional RNA-binding protein with a mass of approximately 82 kDa. This protein participates in various cellular processes by binding to RNA molecules influencing their processing and stability. It is expressed in numerous tissues including hematopoietic cells in the bone marrow and fetal liver. The characteristic expression indicates its importance in developmental and cellular contexts.
- Biological function summary
Rbm15 plays a role in the regulation of mRNA splicing and transcriptional activation. It functions as part of the methyltransferase complex which involves the methylation of mRNA as a post-transcriptional modification contributing to RNA stability and translation efficiency. This action suggests its involvement in gene expression regulation and cellular differentiation.
- Pathways
Rbm15 interacts closely with the Notch signaling pathway which is significant for cell fate determination and differentiation. Rbm15 partners with other proteins such as Numb to mediate repression or activation of Notch-related transcriptional activity. Also Rbm15 affects signaling pathways through interactions with Wnt proteins which play an important role in embryonic development and cell proliferation.
- Associated diseases and disorders
Rbm15 has associations with acute megakaryoblastic leukemia (AMKL) and myelodysplastic syndromes (MDS). In AMKL Rbm15 forms a fusion protein with MKL1 resulting from chromosomal translocations which contributes to leukemogenesis. Understanding its interaction with MKL1 and other fusion partners provides insight into the pathogenesis of these hematological disorders. The study of Rbm15's role in these contexts holds potential for therapeutic advances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunocytochemistry/ Immunofluorescence - Anti-Rbm15/OTT antibody (ab244374)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Rbm15/OTT (green) using ab244374 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rbm15/OTT antibody (ab244374)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rbm15/OTT antibody (ab244374)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rbm15/OTT antibody (ab244374)
Formalin-fixed, paraffin-embedded human testis tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rbm15/OTT antibody (ab244374)
Formalin-fixed, paraffin-embedded human placenta tissue stained for Rbm15/OTT with ab244374 at a 1/20 dilution in immunohistochemical analysis.
Western blot - Anti-Rbm15/OTT antibody (ab244374)
All lanes: Western blot - Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/mL
All lanes: K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 107 kDa
Western blot - Anti-Rbm15/OTT antibody (ab244374)
All lanes: Western blot - Anti-Rbm15/OTT antibody (ab244374) at 0.4 µg/mL
Lane 1: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 2: NBT-II (Rat cell line) cell lysate
Predicted band size: 107 kDa
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