AB70549

Anti-Rbm15/OTT antibody

Name: Anti-Rbm15/OTT antibody (ab70549) | Abcam
Brand: Abcam Limited
SKU: AB70549
Price: 545 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Rbm15/OTT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

OTT, OTT1, RBM15, RNA-binding protein 15, One-twenty two protein 1, RNA-binding motif protein 15

2 Images

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Western blot - Anti-Rbm15/OTT antibody (AB70549)

All lanes:

Western blot - Anti-Rbm15/OTT antibody (ab70549) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 107 kDa

Observed band size: 107 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-Rbm15/OTT antibody (AB70549)

Detection of Human Rbm15/OTT by Western Blot of Immunprecipitate. ab70549 at 1μg/ml staining Rbm15/OTT in HeLa whole cell lysate immunoprecipitated by ab70549 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

All lanes:

Immunoprecipitation - Anti-Rbm15/OTT antibody (ab70549)

Predicted band size: 107 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rbm15 also known as OTT is a multifunctional RNA-binding protein with a mass of approximately 82 kDa. This protein participates in various cellular processes by binding to RNA molecules influencing their processing and stability. It is expressed in numerous tissues including hematopoietic cells in the bone marrow and fetal liver. The characteristic expression indicates its importance in developmental and cellular contexts.

Biological function summary

Rbm15 plays a role in the regulation of mRNA splicing and transcriptional activation. It functions as part of the methyltransferase complex which involves the methylation of mRNA as a post-transcriptional modification contributing to RNA stability and translation efficiency. This action suggests its involvement in gene expression regulation and cellular differentiation.

Pathways

Rbm15 interacts closely with the Notch signaling pathway which is significant for cell fate determination and differentiation. Rbm15 partners with other proteins such as Numb to mediate repression or activation of Notch-related transcriptional activity. Also Rbm15 affects signaling pathways through interactions with Wnt proteins which play an important role in embryonic development and cell proliferation.

Rbm15 has associations with acute megakaryoblastic leukemia (AMKL) and myelodysplastic syndromes (MDS). In AMKL Rbm15 forms a fusion protein with MKL1 resulting from chromosomal translocations which contributes to leukemogenesis. Understanding its interaction with MKL1 and other fusion partners provides insight into the pathogenesis of these hematological disorders. The study of Rbm15's role in these contexts holds potential for therapeutic advances.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed : 27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (By similarity). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed : 27602518). Involved in random X inactivation mediated by Xist RNA : acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed : 27602518). Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (By similarity). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation (PubMed : 26575292). Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen (By similarity). Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL (By similarity). May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing (PubMed : 17001072, PubMed : 19786495). High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing (PubMed : 17001072, PubMed : 19786495). May be implicated in HOX gene regulation (PubMed : 11344311).

See full target information RBM15

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37686120

2023

Human lncRNA Regulates the Proinflammatory Response of Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Yeong-Hwan Lim,Gwangho Yoon,Yeongseo Ryu,Dahee Jeong,Juhyun Song,Yong Sook Kim,Youngkeun Ahn,Hyun Kook,Young-Kook Kim

Developmental cell 57:1466-1481.e6 PubMed35659339

2022

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N-methyladenosine.

Applications

Unspecified application

Species

Unspecified reactive species

Deguan Lv,Ryan C Gimple,Cuiqing Zhong,Qiulian Wu,Kailin Yang,Briana C Prager,Bhaskar Godugu,Zhixin Qiu,Linjie Zhao,Guoxin Zhang,Deobrat Dixit,Derrick Lee,Jia Z Shen,Xiqing Li,Qi Xie,Xiuxing Wang,Sameer Agnihotri,Jeremy N Rich

The Journal of biological chemistry 297:101248 PubMed34582888

2021

Wilms' tumor 1-associating protein complex regulates alternative splicing and polyadenylation at potential G-quadruplex-forming splice site sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Horiuchi,Takeshi Kawamura,Takao Hamakubo

Nature communications 10:2950 PubMed31270318

2019

PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Yu Wang,David Colognori,Hongjae Sunwoo,Danni Wang,Jeannie T Lee

Genes & development 32:415-429 PubMed29535189

2018

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the mA machinery component Wtap/Fl(2)d.

Applications

Unspecified application

Species

Unspecified reactive species

Philip Knuckles,Tina Lence,Irmgard U Haussmann,Dominik Jacob,Nastasja Kreim,Sarah H Carl,Irene Masiello,Tina Hares,Rodrigo Villaseñor,Daniel Hess,Miguel A Andrade-Navarro,Marco Biggiogera,Mark Helm,Matthias Soller,Marc Bühler,Jean-Yves Roignant

Experimental and therapeutic medicine 15:2563-2568 PubMed29456659

2018

PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shuiling Jin,Yanfang Mi,Jing Song,Peipei Zhang,Yanyan Liu

The Journal of biological chemistry 288:33292-302 PubMed24100041

2013

Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle.

Applications

ICC/IF, WB

Species

Human, Human

Keiko Horiuchi,Takeshi Kawamura,Hiroko Iwanari,Riuko Ohashi,Makoto Naito,Tatsuhiko Kodama,Takao Hamakubo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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