Anti-Rbm15/OTT antibody [EPR28158-87]

• WB

Supplier Data

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (AB315456)

The identity of the lower MW bands are unknown.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

Exposure time : Lanes 1-4 : 92 seconds; Lane 5 : 59 seconds;

All lanes:

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (ab315456) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 5:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 107 kDa

false

• WB

Supplier Data

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (AB315456)

The identity of the lower MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 48 seconds; Lane 3 : 70 seconds.

All lanes:

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (ab315456) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 2:

293T transfected with siRNA specifically targeting Rbm15/OTT whole cell lysate at 20 µg with 5% NFDM/TBST

Lane 3:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 107 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (AB315456)

Low expression : liver, brain.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

The identity of the lower MW bands are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (ab315456) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 2:

Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

Lane 3:

Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST

Lane 4:

Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

Lane 5:

Rat liver tissue lysate at 20 µg with 5% NFDM/TBST

Lane 6:

Rat testis tissue lysate at 20 µg with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 107 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (AB315456)

This antibody does not cross-react with human RBM15B.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Rbm15/OTT antibody [EPR28158-87] (ab315456) at 1/1000 dilution

Lane 1:

His-tagged human RBM15 recombinant protein at 10 ng with 5% NFDM/TBST

Lane 2:

His-tagged human RBM15B recombinant protein at 10 ng with 5% NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 24 kDa,27 kDa

false

Exposure time: 15s