AB300347

Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal RBM15B antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

OTT3, RBM15B, Putative RNA-binding protein 15B, One-twenty two protein 3, RNA-binding motif protein 15B, HsOTT3, HuOTT3, OTT, OTT1, RBM15, RNA-binding protein 15, One-twenty two protein 1, RNA-binding motif protein 15

4 Images

Supplier Data

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution

Lane 1:

His-tagged human RBM15B recombinant protein

Lane 2:

His-tagged human RBM15 recombinant protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 25 kDa,20 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lane 4:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution

Lane 1:

Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate

Lane 2:

Hela transfected with siRNA specifically targeti RBM15B, whole cell lysate a 20

Lane 3:

Hela transfected with siRNA specifically targeti RBM15B, whole cell lysate b 20

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa

false

Exposure time: 114s

Supplier Data

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (ab300347) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 2:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 3:

2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 100 kDa

false

Exposure time: 92s

Carrier free

Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25177-138

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rbm15 also known as OTT and RBM15B are members of the RNA-binding motif protein family. Rbm15 has a molecular mass of approximately 101 kDa. Both proteins possess RNA-binding motifs that mediate their involvement in post-transcriptional regulation. Rbm15 and RBM15B are widely expressed in various tissues with notable expression in hematopoietic cells. They regulate RNA methylation by associating with the m6A methyltransferase complex influencing mRNA processing and nuclear export.

Biological function summary

Rbm15 and RBM15B play significant roles in hematopoiesis and embryonic development. They are known to be components of the m6A methylation complex which modifies adenosine residues on RNA affecting RNA stability and translation. This complex interaction enables them to participate in controlling cell differentiation and growth important in maintaining proper cellular function and development. Through their RNA binding ability they also influence splice site selection and alternative splicing of pre-mRNA which is essential in the generation of protein diversity.

Pathways

Both Rbm15 and RBM15B contribute significantly to the maintenance of the Notch signaling and Wnt/β-catenin signaling pathways. These pathways are central to cell fate determination proliferation and apoptosis. Rbm15 interacts with key proteins like Notch receptors promoting signal mediation and thereby affecting downstream gene expression. In the Wnt pathway RBM15B may interface with β-catenin to regulate transcriptional activity influencing cell cycle and differentiation processes alongside proteins such as LEF/TCF.

Rbm15 and RBM15B have connections with acute myeloid leukemia (AML) and developmental disorders. Rbm15 is known for its implication in AML through fusion with the MKL1 gene contributing to leukemogenesis by altering gene expression profiles. Abnormal m6A methylation patterns involving RBM15B can disrupt normal cell differentiation and have been linked to developmental anomalies. These proteins' interactions with disease-related proteins like MKL1 and altered pathways illustrate their importance in understanding disease mechanisms and highlight potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed : 16129689, PubMed : 27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 27602518). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed : 27602518). Involved in random X inactivation mediated by Xist RNA : acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed : 27602518). Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation (PubMed : 16129689). Inhibits pre-mRNA splicing (PubMed : 21044963). Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1 (PubMed : 19586903).

See full target information RBM15B

Additional targets

RBM15

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138]

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Western blot - Anti-Rbm15/OTT + RBM15B antibody [EPR25177-138] (AB300347)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Product promise