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Rabbit Monoclonal RBM15B antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (AB300468), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (AB300468), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (AB300468), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (AB300468), expandable thumbnail
  • Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (AB300468), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)ICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Tested
Tested
Expected
Not recommended
Rat
Tested
Not recommended
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

Species
Mouse
Dilution info
-
Notes

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

Species
Rat
Dilution info
-
Notes

We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:16129689, PubMed:27602518). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:27602518). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (PubMed:27602518). Functions in the regulation of alternative or illicit splicing, possibly by regulating m6A methylation (PubMed:16129689). Inhibits pre-mRNA splicing (PubMed:21044963). Also functions as a mRNA export factor by acting as a cofactor for the nuclear export receptor NXF1 (PubMed:19586903).

Alternative names

OTT3, RBM15B, Putative RNA-binding protein 15B, One-twenty two protein 3, RNA-binding motif protein 15B, HsOTT3, HuOTT3

Recommended products

Rabbit Monoclonal RBM15B antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25177-134
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab300468 is a carrier free version of Anti-RBM15B antibody [EPR25177-134] ab300467.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The band below 100 kDa may be caused by degradation.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Western blot - Anti-RBM15B antibody [EPR25177-134] (Anti-RBM15B antibody [EPR25177-134] ab300467) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

    Lane 2: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lane 3: 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 97 kDa

    Observed band size: 100 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse pancreatic car tissue labeling RBM15B with Anti-RBM15B antibody [EPR25177-134] ab300467 at 1/100 (4.72 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in mouse pancreatic carcinoma. The section was incubated with Anti-RBM15B antibody [EPR25177-134] ab300467 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling RBM15B with Anti-RBM15B antibody [EPR25177-134] ab300467 at 1/100 (4.72 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in mouse cerebrum. The section was incubated with Anti-RBM15B antibody [EPR25177-134] ab300467 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling RBM15B with Anti-RBM15B antibody [EPR25177-134] ab300467 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-RBM15B antibody [EPR25177-134] (Anti-RBM15B antibody [EPR25177-134] ab300467) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Performed under reducing conditions.

    Observed band size: 100 kDa

    Exposure time: 81s

  • Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-RBM15B antibody [EPR25177-134] (Anti-RBM15B antibody [EPR25177-134] ab300467) at 1/1000 dilution

    Lane 1: His-tagged human RBM15B recombinant protein

    Lane 2: His-tagged human RBM15 recombinant protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Performed under reducing conditions.

    Observed band size: 25 kDa

    Exposure time: 7.75s

  • Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468), expandable thumbnail

    Western blot - Anti-RBM15B antibody [EPR25177-134] (BSA and Azide free) (ab300468)

    This data was developed using Anti-RBM15B antibody [EPR25177-134] ab300467, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-RBM15B antibody [EPR25177-134] (Anti-RBM15B antibody [EPR25177-134] ab300467) at 1/1000 dilution

    Lane 1: Hela (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate

    Lane 2: Hela transfected with siRNA specifically targeti RBM15B, whole cell lysate a 20

    Lane 3: Hela transfected with siRNA specifically targeti RBM15B, whole cell lysate b 20

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 100 kDa

    Exposure time: 114s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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