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AB214366

Anti-RBM20 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal RBM20 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RBM20 aa 400-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RNA-binding protein 20, RNA-binding motif protein 20, RBM20

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB214366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB214366)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat heart labeling RBM20 with ab214366 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human RBM20 aa 400-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q5T481

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed : 22466703, PubMed : 24960161, PubMed : 26604136, PubMed : 27496873, PubMed : 27531932, PubMed : 29895960, PubMed : 30948719, PubMed : 32840935, PubMed : 34732726, PubMed : 35427468). Acts as a repressor of mRNA splicing : specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed : 24960161, PubMed : 30948719, PubMed : 34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed : 27531932, PubMed : 30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed : 27531932, PubMed : 34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity).
See full target information RBM20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 233:6689-6704 PubMed29215699

2018

LncRNA LINC00880 promotes cell proliferation, migration, and invasion while inhibiting apoptosis by targeting CACNG5 through the MAPK signaling pathway in spinal cord ependymoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Mei Wu,Yong-Jian Wang,Xin-Rui Han,Xin Wen,Shan Wang,Min Shen,Shao-Hua Fan,Juan Zhuang,Zi-Feng Zhang,Qun Shan,Meng-Qiu Li,Bin Hu,Chun-Hui Sun,Jun Lu,Yuan-Lin Zheng
View all publications
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Product promise

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