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AB233147

Anti-RBM20 antibody

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(5 Publications)

Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody detecting RBM20 in Western Blot, IHC-P. Suitable for Human, Pig.

View Alternative Names

RNA-binding protein 20, RNA-binding motif protein 20, RBM20

3 Images
Western blot - Anti-RBM20 antibody (AB233147)
  • WB

Supplier Data

Western blot - Anti-RBM20 antibody (AB233147)

All lanes:

Western blot - Anti-RBM20 antibody (ab233147) at 2 µg/mL

All lanes:

Recombinant human RBM20 protein

Predicted band size: 134 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB233147)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (AB233147)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RBM20 using ab233147 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-RBM20 antibody (AB233147)
  • WB

Supplier Data

Western blot - Anti-RBM20 antibody (AB233147)

All lanes:

Western blot - Anti-RBM20 antibody (ab233147) at 1.5 µg/mL

All lanes:

Pig skeletal muscle lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Pig, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM20 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5T481

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Pig samples.

What is the molecular weight of RBM20?
Anti-RBM20 (ab233147) specifically detects a band for RBM20 (UniProt: Q5T481) at a molecular weight of 134kDa.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab233147 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed : 22466703, PubMed : 24960161, PubMed : 26604136, PubMed : 27496873, PubMed : 27531932, PubMed : 29895960, PubMed : 30948719, PubMed : 32840935, PubMed : 34732726, PubMed : 35427468). Acts as a repressor of mRNA splicing : specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed : 24960161, PubMed : 30948719, PubMed : 34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed : 27531932, PubMed : 30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed : 27531932, PubMed : 34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity).
See full target information RBM20
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular research 121:629-642 PubMed40042822

2025

Antisense-mediated regulation of exon usage in the elastic spring region of Titin modulates sarcomere function.

Applications

Unspecified application

Species

Unspecified reactive species

Selvi Celik,Ludvig Hyrefelt,Tomasz Czuba,Yuan Li,Juliana Assis,Julia Martinez,Markus Johansson,Oscar André,Jane Synnergren,Joakim Sandstedt,Pontus Nordenfelt,Kristina Vukusic,J Gustav Smith,Olof Gidlöf

Asia Pacific allergy 14:97-102 PubMed39220569

2024

Enhanced IL-37-IL-1R8 axis is negatively associated with inflammatory and clinical severity of chronic rhinosinusitis with nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yujie Cao,Kun Chen,Xianting Hu,Chun Zhou,Lei Li,Miaomiao Han,Dehui Wang,Huabin Li

Nature communications 14:4312 PubMed37463913

2023

Mislocalization of pathogenic RBM20 variants in dilated cardiomyopathy is caused by loss-of-interaction with Transportin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Kornienko,Marta Rodríguez-Martínez,Kai Fenzl,Florian Hinze,Daniel Schraivogel,Markus Grosch,Brigit Tunaj,Dominik Lindenhofer,Laura Schraft,Moritz Kueblbeck,Eric Smith,Chad Mao,Emily Brown,Anjali Owens,Ardan M Saguner,Benjamin Meder,Victoria Parikh,Michael Gotthardt,Lars M Steinmetz

Molecular cell 82:2069-2083.e8 PubMed35427468

2022

Proteome-wide quantitative RNA-interactome capture identifies phosphorylation sites with regulatory potential in RBM20.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Henrique Vieira-Vieira,Vita Dauksaite,Anje Sporbert,Michael Gotthardt,Matthias Selbach

Nature communications 12:6324 PubMed34732726

2021

Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Aidan M Fenix,Yuichiro Miyaoka,Alessandro Bertero,Steven M Blue,Matthew J Spindler,Kenneth K B Tan,Juan A Perez-Bermejo,Amanda H Chan,Steven J Mayerl,Trieu D Nguyen,Caitlin R Russell,Paweena P Lizarraga,Annie Truong,Po-Lin So,Aishwarya Kulkarni,Kashish Chetal,Shashank Sathe,Nathan J Sniadecki,Gene W Yeo,Charles E Murry,Bruce R Conklin,Nathan Salomonis
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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