Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody detecting RBM20 in Western Blot, IHC-P. Suitable for Human, Pig.
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- Molecular cell 82:2069-2083.e82022Proteome-wide quantitative RNA-interactome capture identifies phosphorylation sites with regulatory potential in RBM20.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Henrique Vieira-Vieira,Vita Dauksaite,Anje Sporbert,Michael Gotthardt,Matthias SelbachPubMed 35427468
- Nature communications 12:63242021Gain-of-function cardiomyopathic mutations in RBM20 rewire splicing regulation and re-distribute ribonucleoprotein granules within processing bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAidan M Fenix,Yuichiro Miyaoka,Alessandro Bertero,Steven M Blue,Matthew J Spindler,Kenneth K B Tan,Juan A Perez-Bermejo,Amanda H Chan,Steven J Mayerl,Trieu D Nguyen,Caitlin R Russell,Paweena P Lizarraga,Annie Truong,Po-Lin So,Aishwarya Kulkarni,Kashish Chetal,Shashank Sathe,Nathan J Sniadecki,Gene W Yeo,Charles E Murry,Bruce R Conklin,Nathan SalomonisPubMed 34732726
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RBM20 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T481
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, CAMK2D or PDLIM5/ENH (PubMed:22466703, PubMed:24960161, PubMed:26604136, PubMed:27496873, PubMed:27531932, PubMed:29895960, PubMed:30948719, PubMed:32840935, PubMed:34732726, PubMed:35427468). Acts as a repressor of mRNA splicing: specifically binds the 5'UCUU-3' motif that is predominantly found within intronic sequences of pre-mRNAs, leading to the exclusion of specific exons in target transcripts (PubMed:24960161, PubMed:30948719, PubMed:34732726). RBM20-mediated exon skipping is hormone-dependent and is essential for TTN isoform transition in both cardiac and skeletal muscles (PubMed:27531932, PubMed:30948719). RBM20-mediated exon skipping of TTN provides substrates for the formation of circular RNA (circRNAs) from the TTN transcripts (PubMed:27531932, PubMed:34732726). Together with RBM24, promotes the expression of short isoforms of PDLIM5/ENH in cardiomyocytes (By similarity).
Alternative names
RNA-binding protein 20, RNA-binding motif protein 20, RBM20
Recommended products
Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody detecting RBM20 in Western Blot, IHC-P. Suitable for Human, Pig.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RBM20 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q5T481
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
ab233147 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-RBM20 antibody (ab233147) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Pig samples.
What is the molecular weight of RBM20?
Anti-RBM20 (ab233147) specifically detects a band for RBM20 (UniProt: Q5T481) at a molecular weight of 134kDa.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-RBM20 antibody (ab233147)
All lanes: Western blot - Anti-RBM20 antibody (ab233147) at 2 µg/mL
All lanes: Recombinant human RBM20 protein
Predicted band size: 134 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM20 antibody (ab233147)
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for RBM20 using ab233147 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-RBM20 antibody (ab233147)
All lanes: Western blot - Anti-RBM20 antibody (ab233147) at 1.5 µg/mL
All lanes: Pig skeletal muscle lysate
SecondaryAll lanes: HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 134 kDa
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