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AB150800

Anti-RBM28 antibody

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(1 Publication)

Rabbit Polyclonal RBM28 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBM28 aa 500 to C-terminus.

View Alternative Names

RNA-binding protein 28, RNA-binding motif protein 28, RBM28

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150800)

Immunofluorescent analysis of A431 cells labeling RBM28 with ab150800 antibody at a dilution 4 µg/ml. Fixation/Permeabilization : PFA/Triton X-100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150800)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150800)

Immunohistochemical analysis of paraffin embedded Human urinary bladder tissue labeling RBM28 with ab150800 antibody at a dilution of 1/20.

Western blot - Anti-RBM28 antibody (AB150800)
  • WB

Unknown

Western blot - Anti-RBM28 antibody (AB150800)

All lanes:

Western blot - Anti-RBM28 antibody (ab150800) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM28 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NW13

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM28 also known as RNA-binding motif protein 28 weighs approximately 97 kDa. This protein resides primarily in the nucleolus of cells where it plays key mechanical roles in RNA processing events. As an RNA-binding protein RBM28 binds specifically to snoRNA pre-rRNA and possibly other nucleolar transcripts. It is expressed in various tissues with high expression levels in the brain heart and skeletal muscles indicating its widespread importance in cellular functions.
Biological function summary

RBM28 functions as an essential component of the ribonucleoprotein complexes. It associates with small nucleolar RNAs and pre-ribosomal particles facilitating the maturation of rRNA and the assembly of ribosomes. By engaging in these processes RBM28 ensures the proper synthesis and assembly of ribosomal subunits which are important for protein synthesis in cells. The protein partners with others such as NOP56 and FBL in processing pre-rRNA to mature forms.

Pathways

RBM28 plays a significant role in ribosome biogenesis and RNA metabolic processing. Its involvement in the biogenesis of ribosomes places it within the broader gene expression pathway. The protein interacts with others like DDX21 and nucleolin within this pathway showing its intricate connection to the production and maturation of ribosomes. Such associations help maintain the balance of ribosome production ensuring cells adapt and respond to their translational needs.

RBM28 mutations or dysfunctions relate to conditions like alopecia and neurological disorders. Notably mutations in RBM28 have been linked to alopecia neurological defects and endocrinopathy syndrome indicating the protein's role in hair brain and endocrine system function. The protein's interactions with nucleolin and FBL suggest it might influence developmental pathways affecting neurological and hair follicle homeostasis. Understanding these connections can help in deciphering pathophysiological mechanisms underlying these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nucleolar component of the spliceosomal ribonucleoprotein complexes.
See full target information RBM28
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 144:3659-3673 PubMed28935708

2017

Unraveling transcriptome dynamics in human spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Z Jan,Tinke L Vormer,Aldo Jongejan,Michael D Röling,Sherman J Silber,Dirk G de Rooij,Geert Hamer,Sjoerd Repping,Ans M M van Pelt
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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