JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB150822

Anti-RBM28 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal RBM28 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBM28 aa 350-500.

View Alternative Names

RNA-binding protein 28, RNA-binding motif protein 28, RBM28

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150822)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM28 antibody (AB150822)

Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labelling RBM28 with ab150822 at 1/50 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150822)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150822)

Immunofluorescent analysis of Human cell line U-2 OS, labelling RBM28 with ab150822 at 1/50 (green). Red staining marks microtubules labelled with an anti-tubulin antibody.

Western blot - Anti-RBM28 antibody (AB150822)
  • WB

Unknown

Western blot - Anti-RBM28 antibody (AB150822)

All lanes:

Western blot - Anti-RBM28 antibody (ab150822) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

IgG/HSA-depleted Human plasma

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 85 kDa

false

Western blot - Anti-RBM28 antibody (AB150822)
  • WB

Unknown

Western blot - Anti-RBM28 antibody (AB150822)

Lane 1 : NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)

All lanes:

Western blot - Anti-RBM28 antibody (ab150822)

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM28 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q9NW13

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

ab150822 should be gently mixed before use.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM28 also known as RNA-binding motif protein 28 weighs approximately 97 kDa. This protein resides primarily in the nucleolus of cells where it plays key mechanical roles in RNA processing events. As an RNA-binding protein RBM28 binds specifically to snoRNA pre-rRNA and possibly other nucleolar transcripts. It is expressed in various tissues with high expression levels in the brain heart and skeletal muscles indicating its widespread importance in cellular functions.
Biological function summary

RBM28 functions as an essential component of the ribonucleoprotein complexes. It associates with small nucleolar RNAs and pre-ribosomal particles facilitating the maturation of rRNA and the assembly of ribosomes. By engaging in these processes RBM28 ensures the proper synthesis and assembly of ribosomal subunits which are important for protein synthesis in cells. The protein partners with others such as NOP56 and FBL in processing pre-rRNA to mature forms.

Pathways

RBM28 plays a significant role in ribosome biogenesis and RNA metabolic processing. Its involvement in the biogenesis of ribosomes places it within the broader gene expression pathway. The protein interacts with others like DDX21 and nucleolin within this pathway showing its intricate connection to the production and maturation of ribosomes. Such associations help maintain the balance of ribosome production ensuring cells adapt and respond to their translational needs.

RBM28 mutations or dysfunctions relate to conditions like alopecia and neurological disorders. Notably mutations in RBM28 have been linked to alopecia neurological defects and endocrinopathy syndrome indicating the protein's role in hair brain and endocrine system function. The protein's interactions with nucleolin and FBL suggest it might influence developmental pathways affecting neurological and hair follicle homeostasis. Understanding these connections can help in deciphering pathophysiological mechanisms underlying these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Nucleolar component of the spliceosomal ribonucleoprotein complexes.
See full target information RBM28
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38194286

2024

Extended time frame for restoring inner ear function through gene therapy in Usher1G preclinical model.

Applications

Unspecified application

Species

Unspecified reactive species

Ghizlene Lahlou,Charlotte Calvet,François Simon,Vincent Michel,Lauranne Alciato,Baptiste Plion,Jacques Boutet de Monvel,Marie-José Lecomte,Mathieu Beraneck,Christine Petit,Saaid Safieddine
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB150800

Anti-RBM28 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RBM28 antibody (AB150800)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB184725

Anti-VWA8 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-VWA8 antibody - C-terminal (AB184725)

  • 0
  • 0
Primary Antibodies

AB223477

Anti-ZKSCAN3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (AB223477)

  • 0
  • 0
Primary Antibodies

AB83893

Anti-TREX2 antibody

Western blot - Anti-TREX2 antibody (AB83893)

  • 0
  • 0
Primary Antibodies

AB188075

Anti-TCFL5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCFL5 antibody (AB188075)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com