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AB321825

Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector)

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Knockout Tested Rabbit Recombinant Monoclonal RBM3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

RNPL, RBM3, RNA-binding protein 3, RNA-binding motif protein 3

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Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)

Interpolated concentration of native RBM3 was measured at different sample concentrations. Undiluted samples are as follows : HEK293T cell extract (62.5 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)

Sandwich ELISA of ab321823 with the capture antibody ab321824 dilution at 2 ug/mL and detector antibody ab321825 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)

Interpolated concentration of native RBM3 was measured at different sample concentrations. Undiluted samples are as follows : Jurkat cell extract (250 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) (AB321825)

Interpolated concentrations of native RBM3 in human control wild type HEK293T (human embryonic kidney epithelial cell) and RBM3 knockout HEK293T cell based on 62.5 ug/mL extract loads. The concentrations of RBM3 were measured in duplicate and interpolated from the RBM3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBM3 concentration was determined to be 5022.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in RBM3 knockout HEK293T extract (Human RBM3 knockout HEK293T cell line ab266663).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26597-12

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab321825 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RBM3. The recommended pair for sandwich ELISA is:

Capture: ab321824, Human RBM3 Capture Antibody (unconjugated)

Detector: ab321825, Human RBM3 Detector Antibody (unconjugated)

The reference range value is 2.5-40 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM3 also known as RNA-binding motif protein 3 is a cold-inducible protein with a mass of approximately 17 kDa. It plays a role in binding RNA and is widely expressed in tissues including the brain heart and skeletal muscle. Research often highlights its involvement in temperature response implicating its role in cellular adaptation to cold stress. Overexpression of RBM3 has been documented during hypothermic conditions which suggests an adaptive function in extreme environments.
Biological function summary

RBM3 functions by modulating RNA stability and translation impacting cellular stress responses. It forms part of ribonucleoprotein complexes influencing the processing of several RNA species. The protein enhances mRNA stability under stress conditions and promotes cell survival by impacting protein synthesis. RBM3 also participates in synaptic plasticity which is essential for learning and memory showcasing its extensive influence on cellular functioning.

Pathways

RBM3 significantly contributes to neuroprotection and anti-apoptosis pathways. It is closely linked to pathways dealing with stress granules and mRNA stabilization. A known association exists between RBM3 and proteins such as CIRP (cold-inducible RNA-binding protein) which share similar pathways concerning mRNA regulation under cold conditions. This connection highlights RBM3's role in cell survival and stress management.

RBM3 has shown connections to neurodegenerative diseases and cancer. High levels of RBM3 expression correlate with improved outcomes in hypothermia-treated stroke and trauma patients due to its neuroprotective abilities. In cancer RBM3 links with proteins such as HIF-1α (hypoxia-inducible factor-1 alpha) where its overexpression has been associated with tumor progression in certain types suggesting RBM3's dual protective and pathological roles depending on the disease context.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity).
See full target information RBM3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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