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Knockout Tested Rabbit Recombinant Monoclonal RBM3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (AB321824), expandable thumbnail
  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (AB321824), expandable thumbnail
  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (AB321824), expandable thumbnail
  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (AB321824), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity).

Alternative names

RNPL, RBM3, RNA-binding protein 3, RNA-binding motif protein 3

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal RBM3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26597-3
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab321824 is a BSA and Azide free antibody supplied in an unconjugated
format and it is suitable for sandwich ELISAs to quantify Human RBM3.
The recommended pair for sandwich ELISA is:

Capture: ab321824, Human RBM3 Capture Antibody (unconjugated)

Detector: Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) ab321825, Human RBM3 Detector Antibody (unconjugated)

The reference range value is 2.5-40 ng/mL.

The recommended antibody orientation is based on internal optimization
for ELISAbased assays. Antibody orientation is assay dependent and
needs to be optimized for each assay type. Please note that the
range provided for this antibody is only an estimation based on the
performance of the product using the recommended antibody pair.
Performance of the antibody pair will depend on the specific
characteristics of your assay. We guarantee the product works in
sandwich ELISA, but we do not guarantee the sensitivity or dynamic
range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA,
sodium azide and glycerol for higher conjugation efficiency.
The antibodies are provided at an approximate concentration of
1 mg/ml as measured by the protein A280 method. Use our conjugation
kits for antibody conjugates that are ready-to-use in as
little as 20 minutes with <1 minute hands-on-time and 100% antibody
recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RBM3 also known as RNA-binding motif protein 3 is a cold-inducible protein with a mass of approximately 17 kDa. It plays a role in binding RNA and is widely expressed in tissues including the brain heart and skeletal muscle. Research often highlights its involvement in temperature response implicating its role in cellular adaptation to cold stress. Overexpression of RBM3 has been documented during hypothermic conditions which suggests an adaptive function in extreme environments.

Biological function summary

RBM3 functions by modulating RNA stability and translation impacting cellular stress responses. It forms part of ribonucleoprotein complexes influencing the processing of several RNA species. The protein enhances mRNA stability under stress conditions and promotes cell survival by impacting protein synthesis. RBM3 also participates in synaptic plasticity which is essential for learning and memory showcasing its extensive influence on cellular functioning.

Pathways

RBM3 significantly contributes to neuroprotection and anti-apoptosis pathways. It is closely linked to pathways dealing with stress granules and mRNA stabilization. A known association exists between RBM3 and proteins such as CIRP (cold-inducible RNA-binding protein) which share similar pathways concerning mRNA regulation under cold conditions. This connection highlights RBM3's role in cell survival and stress management.

Associated diseases and disorders

RBM3 has shown connections to neurodegenerative diseases and cancer. High levels of RBM3 expression correlate with improved outcomes in hypothermia-treated stroke and trauma patients due to its neuroprotective abilities. In cancer RBM3 links with proteins such as HIF-1α (hypoxia-inducible factor-1 alpha) where its overexpression has been associated with tumor progression in certain types suggesting RBM3's dual protective and pathological roles depending on the disease context.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824), expandable thumbnail

    Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824)

    Interpolated concentration of native RBM3 was measured at different sample concentrations. Undiluted samples are as follows: HEK293T cell extract (62.5 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824), expandable thumbnail

    Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824)

    Interpolated concentration of native RBM3 was measured at different sample concentrations. Undiluted samples are as follows: Jurkat cell extract (250 ug/mL). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824), expandable thumbnail

    Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824)

    Interpolated concentrations of native RBM3 in human control wild type HEK293T (human embryonic kidney epithelial cell) and RBM3 knockout HEK293T cell based on 62.5 ug/mL extract loads. The concentrations of RBM3 were measured in duplicate and interpolated from the RBM3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RBM3 concentration was determined to be 5022.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in RBM3 knockout HEK293T extract (Human RBM3 knockout HEK293T cell line Human RBM3 knockout HEK-293T cell line ab266663).

  • Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824), expandable thumbnail

    Sandwich ELISA - Anti-RBM3 antibody [EPR26597-3] - BSA and Azide free (Capture) (ab321824)

    Sandwich ELISA of Human RBM3 Antibody Pair - BSA and Azide free ab321823 with the capture antibody ab321824 dilution at 2 ug/mL and detector antibody Anti-RBM3 antibody [EPR26597-12] - BSA and Azide free (Detector) ab321825 dilution at 0.5 ug/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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