AB106555

Anti-RBM35A + RBM35B antibody [23A7.C9]

Name: Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) | Abcam
Brand: Abcam Limited
SKU: AB106555
Price: 520 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal ESRP1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Esrp1.

View Alternative Names

RBM35A, ESRP1, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A, RNA-binding protein 35A, RBM35B, PP7059, ESRP2, Epithelial splicing regulatory protein 2, RNA-binding motif protein 35B, RNA-binding protein 35B

1 Images

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Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (AB106555)

All lanes:

Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) at 1/1000 dilution

Lane 1:

GFP transfected 293T cell extract at 5 µg

Lane 2:

RBM35A transfected 293T cell extract at 5 µg

Lane 3:

RBM35B transfected 293T cell extract at 5 µg

Secondary

All lanes:

anti-mouse ECL antibody at 1/10000 dilution

Predicted band size: 75 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

23A7.C9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Esrp1.

Q3US41

Specificity

This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab106555 was purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM35A and RBM35B also known as ESRP1 and ESRP2 are RNA-binding proteins that play roles in the regulation of alternative splicing. ESRP1 has a mass of approximately 75 kDa while ESRP2 is slightly larger. These proteins are expressed widely in epithelial tissues. They bind specific RNA sequences influencing exon inclusion or exclusion during pre-mRNA splicing. This action alters the outcome and diversity of proteins produced from the gene impacting various cellular processes.

Biological function summary

RBM35A and RBM35B impact the post-transcriptional regulation of gene expression. They often function as part of larger spliceosomal complexes. By deciding the splicing pattern of mRNA they contribute to the diversity of the proteome especially in epithelial cell types. This diversity is important for cell differentiation and tissue-specific functions. The proteins influence the expression of genes involved in cellular adhesion and signaling pathways which are critical for maintaining epithelial cell features.

Pathways

RBM35A and RBM35B play roles in the epithelial-mesenchymal transition (EMT) and Notch signaling pathways. During EMT these proteins regulate key splicing events that change cell adhesion and mobility. This involves interactions with proteins such as FGFR2 and CD44 which play roles in cellular communication and structure. In the Notch signaling pathway RBM35A and RBM35B influence cell fate decisions through alternative splicing of pathway components.

RBM35A and RBM35B have implications in cancer and fibrosis. Their altered expression or function can contribute to cancer progression by influencing EMT a pathway linked to metastasis. Changes in their activity associate with fibrotic diseases where excessive tissue scarring occurs. The interaction of these proteins with CD44 which is involved in tumor progression and fibrosis highlights their role in these processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (PubMed : 19285943). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (By similarity).

See full target information ESRP1

Additional targets

ESRP2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 234:10809-10818 PubMed30536986

2018

Dynamic changes of urinary proteins in a rat model of acute hypercoagulable state induced by tranexamic acid.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Jing,Zhenhuan Du,Zhang Wen,Bo Jiang,Bixi He

Bioscience reports 38: PubMed30049844

2018

Bleomycin-enhanced alternative splicing of fibroblast growth factor receptor 2 induces epithelial to mesenchymal transition in lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kui-Jun Chen,Qing Li,Chang-Mei Weng,Zhao-Xia Duan,Dong-Dong Zhang,Zhi-Qiang Chen,Jing Chen,Jian-Min Wang

Scientific reports 8:15543 PubMed30341388

2018

Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Linjie Chen,Xiaobai He,Yan Xie,Yapei Huang,Dennis W Wolff,Peter W Abel,Yaping Tu

The EMBO journal 29:3286-300 PubMed20711167

2010

An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Claude C Warzecha,Peng Jiang,Karine Amirikian,Kimberly A Dittmar,Hezhe Lu,Shihao Shen,Wei Guo,Yi Xing,Russ P Carstens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBM35A + RBM35B antibody [23A7.C9]

Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (AB106555)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

Product promise