Mouse Monoclonal ESRP1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Esrp1.
Images
![Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (AB106555), expandable thumbnail](https://content.abcam.com/products/images/rbm35a-rbm35b-antibody-23a7c9-ab106555--western-blot-img112339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (AB106555)")
Publications
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- Journal of cellular physiology 234:10809-108182018Dynamic changes of urinary proteins in a rat model of acute hypercoagulable state induced by tranexamic acid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Jing et. al.PubMed 30536986
- Bioscience reports 38:2018Bleomycin-enhanced alternative splicing of fibroblast growth factor receptor 2 induces epithelial to mesenchymal transition in lung fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKui-Jun Chen et. al.PubMed 30049844
- Scientific reports 8:155432018Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinjie Chen et. al.PubMed 30341388
- The EMBO journal 29:3286-3002010An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaude C Warzecha et. al.PubMed 20711167
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Mouse Esrp1. Database link Q3US41
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Horse | Predicted |
| Opossum | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Horse, Cow, Dog, Pig, Chimpanzee, Opossum | Dilution info - | Notes - |
Target data
Function
mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (PubMed:19285943). Regulates splicing and expression of genes involved in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (By similarity).
Additional Targets
ESRP2
Alternative names
RBM35A, ESRP1, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A, RNA-binding protein 35A
Recommended products
Mouse Monoclonal ESRP1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Esrp1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Mouse Esrp1. Database link Q3US41
- Clone number
- 23A7.C9
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
- Concentration
- Purification notes
ab106555 was purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBM35A and RBM35B also known as ESRP1 and ESRP2 are RNA-binding proteins that play roles in the regulation of alternative splicing. ESRP1 has a mass of approximately 75 kDa while ESRP2 is slightly larger. These proteins are expressed widely in epithelial tissues. They bind specific RNA sequences influencing exon inclusion or exclusion during pre-mRNA splicing. This action alters the outcome and diversity of proteins produced from the gene impacting various cellular processes.
- Biological function summary
RBM35A and RBM35B impact the post-transcriptional regulation of gene expression. They often function as part of larger spliceosomal complexes. By deciding the splicing pattern of mRNA they contribute to the diversity of the proteome especially in epithelial cell types. This diversity is important for cell differentiation and tissue-specific functions. The proteins influence the expression of genes involved in cellular adhesion and signaling pathways which are critical for maintaining epithelial cell features.
- Pathways
RBM35A and RBM35B play roles in the epithelial-mesenchymal transition (EMT) and Notch signaling pathways. During EMT these proteins regulate key splicing events that change cell adhesion and mobility. This involves interactions with proteins such as FGFR2 and CD44 which play roles in cellular communication and structure. In the Notch signaling pathway RBM35A and RBM35B influence cell fate decisions through alternative splicing of pathway components.
- Associated diseases and disorders
RBM35A and RBM35B have implications in cancer and fibrosis. Their altered expression or function can contribute to cancer progression by influencing EMT a pathway linked to metastasis. Changes in their activity associate with fibrotic diseases where excessive tissue scarring occurs. The interaction of these proteins with CD44 which is involved in tumor progression and fibrosis highlights their role in these processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555), expandable thumbnail](https://content.abcam.com/products/images/rbm35a-rbm35b-antibody-23a7c9-ab106555--western-blot-img112339.jpg "Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)")
Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)
All lanes: Western blot - Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) at 1/1000 dilution
Lane 1: GFP transfected 293T cell extract at 5 µg
Lane 2: RBM35A transfected 293T cell extract at 5 µg
Lane 3: RBM35B transfected 293T cell extract at 5 µg
SecondaryAll lanes: anti-mouse ECL antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 75 kDa
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