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AB251923

Anti-RBM4 antibody

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(1 Publication)

Rabbit Polyclonal RBM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBM4 aa 300 to C-terminus.

View Alternative Names

RBM4A, RBM4, RNA-binding protein 4, Lark homolog, RNA-binding motif protein 4, RNA-binding motif protein 4a, hLark

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM4 antibody (AB251923)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM4 antibody (AB251923)

Paraffin-embedded human hippocampus tissue stained for RBM4 using ab251923 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RBM4 antibody (AB251923)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBM4 antibody (AB251923)

PFA-fixed, Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for RBM4 (green) using ab251923 at 4 µg/ml in ICC/IF.

Western blot - Anti-RBM4 antibody (AB251923)
  • WB

Unknown

Western blot - Anti-RBM4 antibody (AB251923)

All lanes:

Western blot - Anti-RBM4 antibody (ab251923) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBM4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BWF3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNA Binding Motif Protein 4 (RBM4) also known as LARK1 is a RNA-binding protein with a molecular mass of approximately 37 kDa. This protein plays a role in post-transcriptional regulation by binding to RNA and influencing alternative splicing. RBM4 mainly expresses in the cytoplasm of various tissues including muscle and neuronal tissues and is involved in the regulation of mRNA synthesis and turnover. Its function relies on the presence of RNA recognition motifs that facilitate its binding to RNA targets.
Biological function summary

RNA Binding Motif Protein 4 impacts cellular processes through its ability to modulate alternative splicing and mRNA translation. It has been shown to form complexes with other RNA-binding proteins and splicing factors enhancing its regulatory capabilities. Through these interactions RBM4 can influence the expression of a wide array of genes involved in cellular development and differentiation adapting protein synthesis to meet cellular and environmental cues.

Pathways

RBM4's function contributes significantly to the regulation of insulin signaling and muscle differentiation pathways. By interacting with other proteins such as SRSF1 and SRPK1 RBM4 aids in modulating the gene splicing required for effective insulin response and muscle cell formation. These interactions ensure that the pathways remain responsive to internal cellular signals and external metabolic demands coordinating essential physiological processes.

RNA Binding Motif Protein 4 has been linked to the development of metabolic disorders and certain types of cancer. Aberrant expression or mutations in RBM4 can disrupt normal insulin signaling which may result in type 2 diabetes. In cancer its altered activity can affect cell cycle regulation and apoptosis through connections with other proteins like p53 contributing to tumorigenesis and cancer progression. Understanding the role of RBM4 in these conditions highlights its therapeutic potential as a target for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.
See full target information RBM4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:873382 PubMed35720361

2022

Adiponectin Ameliorates GMH-Induced Brain Injury by Regulating Microglia M1/M2 Polarization AdipoR1/APPL1/AMPK/PPARγ Signaling Pathway in Neonatal Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ningbo Xu,Xifeng Li,Jun Weng,Chunhua Wei,Zhenyan He,Desislava Met Doycheva,Cameron Lenahan,Wenhui Tang,Jian Zhou,Yanchao Liu,Qiang Xu,Yahong Liu,Xuying He,Jiping Tang,John H Zhang,Chuanzhi Duan
View all publications
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