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AB69770

Anti-RBM5 antibody

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(2 Publications)

Rabbit Polyclonal RBM5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RBM5.

View Alternative Names

H37, LUCA15, RBM5, RNA-binding protein 5, Protein G15, Putative tumor suppressor LUCA15, RNA-binding motif protein 5, Renal carcinoma antigen NY-REN-9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM5 antibody (AB69770)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM5 antibody (AB69770)

Left : human brain tissue stained with ab69770 at 1/50 dilution; Right : the antibody was preincubated with the immunizing peptide.

Western blot - Anti-RBM5 antibody (AB69770)
  • WB

Unknown

Western blot - Anti-RBM5 antibody (AB69770)

All lanes:

Western blot - Anti-RBM5 antibody (ab69770) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 15 µg

Lane 2:

Extracts from HUVEC cells at 15 µg with immunizing peptide

Predicted band size: 92 kDa

Observed band size: 31 kDa,92 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human RBM5. The exact immunogen used to generate this antibody is proprietary information.

P52756

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM5 also known as RNA-binding motif protein 5 or LUCA-15 plays a significant role in pre-mRNA splicing regulation. Its molecular mass is approximately 94 kDa. This protein is expressed in various tissues with noticeable levels in the heart liver and lung. RBM5 influences splice site selection during RNA processing impacting gene expression patterns.
Biological function summary

RBM5 functions as a tumor suppressor influencing cell cycle regulation and apoptosis. It forms part of a spliceosomal complex playing a critical role in the alternative splicing of its target mRNAs. This activity is essential for maintaining normal cellular homeostasis especially in rapidly dividing cells. RBM5 interacts with other splicing factors to modulate the inclusion of specific exons impacting overall cell function.

Pathways

The regulation of alternative splicing by RBM5 integrates into the apoptotic and cell cycle pathways. Its interaction with proteins like Fas and Bcl-2 highlights its role in managing programmed cell death. These connections make RBM5 central in ensuring the balance between cell survival and death an important element in cancer suppression. It also collaborates with other splicing proteins in the spliceosomal pathway to coordinate precise RNA maturation.

Mutations or altered expression of RBM5 link to cancer notably lung and breast cancer. Its tumor-suppressive effects in these conditions highlight its importance in maintaining normal cell behavior. Moreover RBM5 interacts with p53 a well-known tumor suppressor protein indicating its coordinated role in preventing aberrant cell proliferation. The dysregulation of RBM5 and subsequent disruption in RNA processing highlights its significance in cancer-related pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate apoptosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis.
See full target information RBM5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:3241-3255 PubMed39072172

2024

RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells stabilizing phosphatase and tensin homolog mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-Xiang Wang,Feng Liu,Yi Wang

Viruses 6:89-105 PubMed24385082

2014

Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Puerta Martínez,Qiyi Tang
View all publications
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