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AB232475

Anti-RBM6 antibody [EPR15842] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RBM6 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DEF3, RBM6, RNA-binding protein 6, Lung cancer antigen NY-LU-12, Protein G16, RNA-binding motif protein 6, RNA-binding protein DEF-3

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBM6 antibody [EPR15842] - BSA and Azide free (AB232475)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBM6 antibody [EPR15842] - BSA and Azide free (AB232475)

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 (human lung carcinoma cell line) cells labeling RBM6 with ab188318 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight® 488) at a 1/200 dilution. Counterstained with DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188318).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15842

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab232475 is the carrier-free version of ab188318.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM6 also known as RNA binding motif protein 6 plays a significant role in RNA binding activities. It exhibits a molecular mass of approximately 94 kDa. This protein is expressed in a variety of tissues with higher expression levels observed in the lung and brain. RBM6 contains several RNA recognition motifs that facilitate its involvement in the regulation of RNA processing including splicing stability and transport.
Biological function summary

RNA binding motif protein 6 contributes to the regulation of gene expression by interacting with specific RNA sequences. It often functions as part of a larger ribonucleoprotein complex. Through its interactions RBM6 influences alternative splicing events which modify mRNA precursors to create diverse protein isoforms. This activity is essential for the cellular response to different physiological conditions and RBM6 has been implicated in cell cycle regulation and apoptosis.

Pathways

RNA binding motif protein 6 is involved in several important cellular processes. It is an important component of the splicing machinery and participates in the spliceosome pathway interacting with other splicing factors like SF3B1 and SRSF2. Additionally RBM6 has links to the RNA transport pathway influencing the export of mature mRNAs from the nucleus to the cytoplasm where translation occurs.

Alterations in RNA binding motif protein 6 can impact oncogenesis and neurological conditions. Abnormal expression of RBM6 has been observed in certain cancers such as lung cancer where it may cooperate with or have effects opposite to tumor suppressor proteins like RBM5. Furthermore mutations or dysregulation of RBM6 might affect neural development or function suggesting a possible connection to neurodegenerative disorders where it could interact with genes involved in neuronal survival and degeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically binds poly(G) RNA homopolymers in vitro.
See full target information RBM6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 17:2062-2071 PubMed36568515

2022

MGST1 alleviates the oxidative stress of trophoblast cells induced by hypoxia/reoxygenation and promotes cell proliferation, migration, and invasion by activating the PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Dai,Xianmei Lu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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