AB188318

Anti-RBM6 antibody [EPR15842] - C-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RBM6 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DEF3, RBM6, RNA-binding protein 6, Lung cancer antigen NY-LU-12, Protein G16, RNA-binding motif protein 6, RNA-binding protein DEF-3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling RBM6 with ab188318at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Dylight® 488) at a 1/200 dilution. Counterstained with Dapi blue.

Supplier Data

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

All lanes:

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 129 kDa

Observed band size: 129 kDa

false

Supplier Data

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

All lanes:

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (ab188318) at 1/5000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 129 kDa

Observed band size: 129 kDa

false

Carrier free

Anti-RBM6 antibody [EPR15842] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15842

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBM6 also known as RNA binding motif protein 6 plays a significant role in RNA binding activities. It exhibits a molecular mass of approximately 94 kDa. This protein is expressed in a variety of tissues with higher expression levels observed in the lung and brain. RBM6 contains several RNA recognition motifs that facilitate its involvement in the regulation of RNA processing including splicing stability and transport.

Biological function summary

RNA binding motif protein 6 contributes to the regulation of gene expression by interacting with specific RNA sequences. It often functions as part of a larger ribonucleoprotein complex. Through its interactions RBM6 influences alternative splicing events which modify mRNA precursors to create diverse protein isoforms. This activity is essential for the cellular response to different physiological conditions and RBM6 has been implicated in cell cycle regulation and apoptosis.

Pathways

RNA binding motif protein 6 is involved in several important cellular processes. It is an important component of the splicing machinery and participates in the spliceosome pathway interacting with other splicing factors like SF3B1 and SRSF2. Additionally RBM6 has links to the RNA transport pathway influencing the export of mature mRNAs from the nucleus to the cytoplasm where translation occurs.

Alterations in RNA binding motif protein 6 can impact oncogenesis and neurological conditions. Abnormal expression of RBM6 has been observed in certain cancers such as lung cancer where it may cooperate with or have effects opposite to tumor suppressor proteins like RBM5. Furthermore mutations or dysregulation of RBM6 might affect neural development or function suggesting a possible connection to neurodegenerative disorders where it could interact with genes involved in neuronal survival and degeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Specifically binds poly(G) RNA homopolymers in vitro.

See full target information RBM6

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:374 PubMed41044623

2025

THBS1 regulates the function and insulin sensitivity of HTR8/SVneo cells treated with high glucose through the RhoA/ROCK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuerong Chai,Jie Tang,Aimin Liu,Qin Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBM6 antibody [EPR15842] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

Western blot - Anti-RBM6 antibody [EPR15842] - C-terminal (AB188318)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise