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AB248936

Anti-RBMS1 antibody [EPR9825(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RBMS1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

C2orf12, MSSP, MSSP1, SCR2, RBMS1, Single-stranded DNA-binding protein MSSP-1, Suppressor of CDC2 with RNA-binding motif 2

1 Images
Western blot - Anti-RBMS1 antibody [EPR9825(B)] - BSA and Azide free (AB248936)
  • WB

Unknown

Western blot - Anti-RBMS1 antibody [EPR9825(B)] - BSA and Azide free (AB248936)

This data was developed using ab150353, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RBMS1 antibody [EPR9825(B)] (<a href='/en-us/products/primary-antibodies/rbms1-antibody-epr9825b-ab150353'>ab150353</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

Raw 264.7 cell lysate at 10 µg

Lane 6:

NIH3T3 cell lysate at 10 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9825(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248936 is the carrier-free version of ab150353.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBMS1 or RNA-binding motif single-stranded interacting protein 1 is a protein that plays an important role in binding single-stranded RNA and DNA. It has a molecular mass of approximately 45 kDa and is widely expressed in various tissues including but not limited to the brain liver and heart. Functionally RBMS1 contributes to a range of cellular processes by regulating the stability and translation of its bound nucleic acids.
Biological function summary

The RNA-binding capabilities of RBMS1 enable it to exert influence over gene expression and cellular proliferation. RBMS1 interacts with multiple other proteins becoming part of larger complexes that facilitate its regulatory actions. Complex formation with diverse molecular partners allows RBMS1 to influence different aspects of RNA metabolism and stability impacting cellular growth and development.

Pathways

The involvement of RBMS1 is notable in pathways regulating cell cycle and apoptosis. RBMS1 associates with components of the cell cycle control machinery affecting checkpoint regulation and cell proliferation. Its interaction with proteins like MYC and other cell cycle pathogenic elements highlights its role in the fine-tuning of growth-related cascades and programmed cell death mechanisms.

RBMS1 is associated with certain types of cancer notably those where gene expression misregulation is a factor. Altered expression or mutation of RBMS1 may contribute to oncogenic processes alongside proteins such as KRAS facilitating tumor progression and metastasis. Furthermore RBMS1 dysregulation can influence neurological disorders possibly through pathways that involve cell death such as those linked to neurodegenerative disease markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.
See full target information RBMS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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