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AB250332

Anti-RBMS3+ RBMS1 antibody [EPR12905] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RBMS1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C2orf12, MSSP, MSSP1, SCR2, RBMS1, Single-stranded DNA-binding protein MSSP-1, Suppressor of CDC2 with RNA-binding motif 2, RBMS3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBMS3+ RBMS1 antibody [EPR12905] - BSA and Azide free (AB250332)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBMS3+ RBMS1 antibody [EPR12905] - BSA and Azide free (AB250332)

This data was developed using ab181082, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of THP1 cells (-20 ° Acetone-fixed) labeling RBMS1 with ab181082 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Dylight 488). Conterstained with Dapi (blue).

Western blot - Anti-RBMS3+ RBMS1 antibody [EPR12905] - BSA and Azide free (AB250332)
  • WB

Supplier Data

Western blot - Anti-RBMS3+ RBMS1 antibody [EPR12905] - BSA and Azide free (AB250332)

This data was developed using ab181082, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RBMS3+ RBMS1 antibody [EPR12905] (<a href='/en-us/products/primary-antibodies/rbms3-rbms1-antibody-epr12905-ab181082'>ab181082</a>) at 1/1000 dilution

Lane 1:

NIH:OVCAR-3 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa,48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12905

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Immunogen also corresponds to amino acids 102 -114 of RBMS3 (Q6XE24).

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Primary Antibodies

AB272612

Anti-RBMS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMS3 antibody (AB272612)

  • 0
  • 0
Primary Antibodies

AB32454

Anti-MAP2 antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody - Neuronal Marker (AB32454)

  • 5
  • 35
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250332 is the carrier-free version of ab181082.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBMS3 and RBMS1 are members of the RNA-binding motif single-stranded interacting protein family known for their role in nucleic acid binding. RBMS3 often referred to as RNA-binding motif single-stranded-interacting protein 3 possesses a molecular mass of approximately 43 kDa. RBMS1 shares a similar domain structure targeted as an RNA-binding protein. These proteins express in various tissues including the liver heart and lungs with significant levels in the brain and kidneys. Both RBMS3 and RBMS1 play a part in regulating cellular proliferation and have been linked to the stabilization of specific mRNA transcripts.
Biological function summary

RBMS3 and RBMS1 take part in processes that impact cell growth apoptosis and differentiation. They involve themselves in RNA metabolism where they can influence mRNA stability and transcription. In terms of complex formation they associate with other factors to regulate transcription and influence gene expression. They help control genes involved in cellular growth responses and have implications for maintaining cellular homeostasis.

Pathways

RBMS3 and RBMS1 contribute to the regulation of the cell cycle and apoptosis. They participate in the Wnt signaling pathway a critical route for cell proliferation and fate determination. In connection with this pathway they interact with proteins such as beta-catenin which is essential for transmitting signals within the Wnt pathway. These proteins also influence other signaling cascades that affect cellular growth and differentiation.

RBMS3 and RBMS1 show significant influence in cancer and fibrosis. Their misregulation associates with oncogenic processes where they can contribute to abnormal cell proliferation linked to various cancers. They interact with p53 a well-known tumor suppressor protein whose pathway mutations are common in cancer types. RBMS3 and RBMS1 have been implicated in tissue fibrosis where improper cell growth and response lead to the pathological development of fibrotic tissues. Their expression patterns and interactions make them significant in understanding complex changes in diseased states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA binding protein that interacts with the region upstream of the MYC gene. Binds specifically to the DNA sequence motif 5'-[AT]CT[AT][AT]T-3'. Probably has a role in DNA replication.
See full target information RBMS1

Additional targets

RBMS3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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