Rabbit Recombinant Monoclonal RBMX2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunocytochemistry-immunofluorescence-img70592.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272)")
![Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--western-blot-img124287.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272)")
![Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--western-blot-img81965.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126768.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33393.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (AB250272)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Involved in pre-mRNA splicing as component of the activated spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Alternative names
CGI-79, RBMX2
Recommended products
Rabbit Recombinant Monoclonal RBMX2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR14394
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250272 is the carrier-free version of Anti-RBMX2 antibody [EPR14394] ab180940.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBMX2 also known as RNA-binding motif protein X-linked 2 (RBMY2 or hnRNP G-T) is a protein that binds to RNA to help regulate gene expression. This protein weighs approximately 40 kDa and is located primarily within the nucleus of cells. RBMX2 shows expression in various tissues with noticeable levels in testis tissue and to some extent in other organs suggesting a role in specific cellular processes.
- Biological function summary
RNA-binding motif proteins like RBMX2 engage in RNA splicing processing and transport thereby influencing RNA metabolism. RBMX2 may be a part of ribonucleoprotein complexes that modulate mRNA processing events. These complexes maintain RNA stability and facilitate efficient gene expression impacting cell differentiation and proliferation.
- Pathways
The RBMX2 protein influences RNA processing pathways. It interacts with other RNA-binding proteins such as DAZL and hnRNPA2/B1 in these pathways. These interactions highlight its involvement in post-transcriptional regulatory networks which control the proper maturation and trafficking of mRNAs essential for cellular functions.
- Associated diseases and disorders
RBMX2's aberrant expression can relate to testicular dysgenesis and reproductive issues. Alterations in RBMX2 functionality have connections with infertility particularly involving the DAZ gene family critical for spermatogenesis. Disruptions in these pathways might lead to improper RNA processing contributing to developmental defects and infertility-related syndromes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunocytochemistry-immunofluorescence-img70592.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining RBMX2 using Anti-RBMX2 antibody [EPR14394] ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).
![Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--western-blot-img124287.jpg "Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-RBMX2 antibody [EPR14394] (Anti-RBMX2 antibody [EPR14394] ab180940) at 1/10000 dilution
Lane 1: HepG2 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
![Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--western-blot-img81965.jpg "Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Western blot - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-RBMX2 antibody [EPR14394] (Anti-RBMX2 antibody [EPR14394] ab180940) at 1/2000 dilution
All lanes: HT-1376 at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126768.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RBMX2 with Anti-RBMX2 antibody [EPR14394] ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33393.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling RBMX2 with Anti-RBMX2 antibody [EPR14394] ab180940 at a 1/100 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunocytochemistry-immunofluorescence-img93623.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)
This data was developed using Anti-RBMX2 antibody [EPR14394] ab180940, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of -20? acetone-fixed HeLa cell line staining RBMX2 using Anti-RBMX2 antibody [EPR14394] ab180940 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. Counter stain: Dapi (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272), expandable thumbnail](https://content.abcam.com/products/images/rbmx2-antibody-epr14394-bsa-and-azide-free-ab250272--immunocytochemistry-immunofluorescence-img93623.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RBMX2 antibody [EPR14394] - BSA and Azide free (ab250272)")
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