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Rabbit Recombinant Monoclonal RBMY1A1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs.

Alternative names

RBM1, RBM2, YRRM1, YRRM2, RBMY1A1, RNA-binding motif protein 1, RNA-binding motif protein 2, Y chromosome RNA recognition motif 1, hRBMY

Recommended products

Rabbit Recombinant Monoclonal RBMY1A1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
R12508(2)
Purification technique
Affinity purification Protein A
Specificity

Based on sequence analysis, this antibody recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251302 is the carrier-free version of Anti-RBMY1A1 antibody [R12508(2)] ab200202.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RBMY1A1 also known as RNA-binding motif protein Y chromosome family 1 member A1 is a protein with a molecular mass of about 47 kDa. It is expressed predominantly in the testis playing an important role in male germ cell development. The protein functions mechanically by binding to RNA featuring an RNA recognition motif that facilitates its role in RNA processing. Its localization to the nucleus highlights its function in post-transcriptional regulation.

Biological function summary

RBMY1A1 contributes to spermatogenesis by participating in the regulation of RNA splicing and stability. It is part of a larger complex of proteins that associate with RNA impacting gene expression in male germ cells. The protein interacts with other splicing factors influencing the alternative splicing of pre-mRNAs essential for spermatogenic processes. This highlights RBMY1A1's specific role in sperm development and functioning.

Pathways

The involvement of RBMY1A1 is significant in the mRNA processing pathway. It connects with proteins like hnRNPGT and other spliceosomal components. RBMY1A1’s interaction in this pathway helps in the formation of messenger ribonucleoprotein complexes which are necessary for the maturation and transport of mRNA molecules. Its activity is important in maintaining the integrity of gene expression regulation in spermatogenic cells intertwining with broader pathways of male fertility.

Associated diseases and disorders

RBMY1A1 has been implicated in cases of azoospermia a condition characterized by the absence of sperm in semen. This condition associates with its critical role in fertility where loss or malfunction correlates with impaired sperm production. Moreover RBMY1A1 has links to testicular germ cell tumors where abnormal expression and mutations have been observed. It also interacts with other proteins like DAZL which is another factor associated with reproductive health emphasizing its relevance in male reproductive disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302), expandable thumbnail

    Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)

    This data was developed using Anti-RBMY1A1 antibody [R12508(2)] ab200202, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Please note lanes 2-5 represent RBMY1A1 negative lysates.

    Based on sequence analysis, Anti-RBMY1A1 antibody [R12508(2)] ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

    All lanes: Western blot - Anti-RBMY1A1 antibody [R12508(2)] (Anti-RBMY1A1 antibody [R12508(2)] ab200202) at 1/1000 dilution

    Lane 1: Human testis lysate at 10 µg

    Lane 2: Human fetal brain tissue lysate at 10 µg

    Lane 3: Human fetal heart tissue lysate at 10 µg

    Lane 4: Human fetal kidney tissue lysate at 10 µg

    Lane 5: Human fetal spleen tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 56 kDa

    Observed band size: 51 kDa, 56 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)

    This data was developed using Anti-RBMY1A1 antibody [R12508(2)] ab200202, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RBMY1A1 with Anti-RBMY1A1 antibody [R12508(2)] ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining of Human testis tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (ab251302)

    This data was developed using Anti-RBMY1A1 antibody [R12508(2)] ab200202, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue with Anti-RBMY1A1 antibody [R12508(2)] ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Please note Human liver tissue represents a RBMY1A1 negative control. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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