AB251302

Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RBMY1A1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RBM1, RBM2, YRRM1, YRRM2, RBMY1A1, RNA-binding motif protein 1, RNA-binding motif protein 2, Y chromosome RNA recognition motif 1, hRBMY

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

This data was developed using ab200202, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Please note Human liver tissue represents a RBMY1A1 negative control.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

This data was developed using ab200202, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RBMY1A1 with ab200202 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining of Human testis tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

This data was developed using ab200202, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Please note lanes 2-5 represent RBMY1A1 negative lysates.

Based on sequence analysis, ab200202 recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

All lanes:

Western blot - Anti-RBMY1A1 antibody [R12508(2)] (<a href='/en-us/products/primary-antibodies/rbmy1a1-antibody-r125082-ab200202'>ab200202</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Lane 5:

Human fetal spleen tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 51 kDa,56 kDa

false

Exposure time: 5s

Unconjugated

Anti-RBMY1A1 antibody [R12508(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

R12508(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on sequence analysis, this antibody recognizes 2 isoforms with the predicted MWs of 56KDa and 51KDa respectively. Sequence analysis also showed a 100% homology with RBY1F, RBY1E, RBY1D, RBY1C and RBY1B.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab251302 is the carrier-free version of ab200202.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBMY1A1 also known as RNA-binding motif protein Y chromosome family 1 member A1 is a protein with a molecular mass of about 47 kDa. It is expressed predominantly in the testis playing an important role in male germ cell development. The protein functions mechanically by binding to RNA featuring an RNA recognition motif that facilitates its role in RNA processing. Its localization to the nucleus highlights its function in post-transcriptional regulation.

Biological function summary

RBMY1A1 contributes to spermatogenesis by participating in the regulation of RNA splicing and stability. It is part of a larger complex of proteins that associate with RNA impacting gene expression in male germ cells. The protein interacts with other splicing factors influencing the alternative splicing of pre-mRNAs essential for spermatogenic processes. This highlights RBMY1A1's specific role in sperm development and functioning.

Pathways

The involvement of RBMY1A1 is significant in the mRNA processing pathway. It connects with proteins like hnRNPGT and other spliceosomal components. RBMY1A1’s interaction in this pathway helps in the formation of messenger ribonucleoprotein complexes which are necessary for the maturation and transport of mRNA molecules. Its activity is important in maintaining the integrity of gene expression regulation in spermatogenic cells intertwining with broader pathways of male fertility.

RBMY1A1 has been implicated in cases of azoospermia a condition characterized by the absence of sperm in semen. This condition associates with its critical role in fertility where loss or malfunction correlates with impaired sperm production. Moreover RBMY1A1 has links to testicular germ cell tumors where abnormal expression and mutations have been observed. It also interacts with other proteins like DAZL which is another factor associated with reproductive health emphasizing its relevance in male reproductive disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein involved in pre-mRNA splicing. Required for sperm development. Acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN. Binds non-specifically to mRNAs.

See full target information RBMY1A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

Western blot - Anti-RBMY1A1 antibody [R12508(2)] - BSA and Azide free (AB251302)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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