AB307683

Anti-RBP1 antibody [42/RBP]

Recombinant
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Mouse Recombinant Monoclonal RET1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I

2 Images

Supplier Data

Western blot - Anti-RBP1 antibody [42/RBP] (AB307683)

Lanes 1, 2, 3, 4 and 6 : 180 seconds; Lane 5 : 48 seconds. Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RBP1 antibody [42/RBP] (ab307683) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Mouse pancreas tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Rat pancreas tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 16 kDa

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Supplier Data

Western blot - Anti-RBP1 antibody [42/RBP] (AB307683)

Blocking buffer : 5% NFDM/TBST. Exposure time : 180 seconds. Low expression : MCF7 and PANC-1 (PMID : 24982334; PMID : 12384555) In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-RBP1 antibody [42/RBP] (ab307683) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 16 kDa

false

Exposure time: 180s

Carrier free

Anti-RBP1 antibody [42/RBP] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

42/RBP

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinol binding protein 1 (RBP1) also known as cellular retinol-binding protein 1 (CRBP1) plays a critical role in the regulation and transportation of retinol a form of vitamin A within cells. RBP1 has a molecular mass of approximately 15 kDa and localizes predominantly in the cytoplasm. It is expressed in multiple tissues including the liver kidney and adipose tissue where it helps in regulating retinoid metabolism. The presence of RBP1 in these tissues is essential for maintaining physiological retinol levels.

Biological function summary

RBP1 assists in the cellular uptake storage and metabolism of retinol facilitating its conversion into retinal and retinoic acid. Although it is not part of a well-defined complex RBP1 works closely with enzymes involved in retinol metabolism such as lecithin retinol acyltransferase (LRAT) and retinal dehydrogenases. This process is important for maintaining visual function immune response and cellular differentiation which all rely on proper retinol metabolism and activity.

Pathways

RBP1 plays a significant role in the retinoic acid signaling pathway affecting gene expression regulation by modulating retinoid concentrations. It interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) pathways influencing transcriptional activities related to development and differentiation. Connections between RBP1 and LRAT are important for the retinol esterification process integrating into broader retinoid metabolism and signaling pathways that govern cellular functions.

Dysregulation of RBP1 can contribute to conditions such as age-related macular degeneration (AMD) and metabolic syndrome. In AMD impaired retinol transport and metabolism lead to retinal damage and vision loss. In metabolic syndrome altered retinoid homeostasis involving RBP1 may exacerbate insulin resistance and obesity. Relationships with proteins like LRAT and retinaldehyde dehydrogenase (RALDH) highlight its involvement in disease mechanisms related to retinol dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytoplasmic retinol-binding protein (PubMed : 22665496, PubMed : 26900151, PubMed : 28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed : 15632377, PubMed : 22665496).

See full target information RBP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBP1 antibody [42/RBP]

Western blot - Anti-RBP1 antibody [42/RBP] (AB307683)

Western blot - Anti-RBP1 antibody [42/RBP] (AB307683)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise