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AB104452

Anti-RBP1 antibody

5

(1 Review)

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(1 Publication)

Goat Polyclonal RET1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RBP1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)

ab104452, at 5 µg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Pancreas by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)

ab104452, at 5 μg/ml, staining RBP1 in formalin-fixed, paraffin-embedded Human Liver by Immunohistochemistry, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)
  • IHC-P

AbReview30467****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (AB104452)

ab104452 staining mouse liver sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab104452 at 1/100 in TBS/BSA/azide for 2h at 21°C. A biotinylated rabbit anti-goat polyclonal antibody at 1/250 was used as the secondary antibody. Red arrowheads indicate strongly stained Kupffer cells.

This image is courtesy of an Abreview submitted by Carl Hobbs

Western blot - Anti-RBP1 antibody (AB104452)
  • WB

Unknown

Western blot - Anti-RBP1 antibody (AB104452)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-RBP1 antibody (ab104452) at 0.1 µg/mL

All lanes:

NIH 3T3 lysate (in RIPA buffer) at 35 µg

Predicted band size: 16 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RBP1 aa 100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P09455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinol binding protein 1 (RBP1) also known as cellular retinol-binding protein 1 (CRBP1) plays a critical role in the regulation and transportation of retinol a form of vitamin A within cells. RBP1 has a molecular mass of approximately 15 kDa and localizes predominantly in the cytoplasm. It is expressed in multiple tissues including the liver kidney and adipose tissue where it helps in regulating retinoid metabolism. The presence of RBP1 in these tissues is essential for maintaining physiological retinol levels.
Biological function summary

RBP1 assists in the cellular uptake storage and metabolism of retinol facilitating its conversion into retinal and retinoic acid. Although it is not part of a well-defined complex RBP1 works closely with enzymes involved in retinol metabolism such as lecithin retinol acyltransferase (LRAT) and retinal dehydrogenases. This process is important for maintaining visual function immune response and cellular differentiation which all rely on proper retinol metabolism and activity.

Pathways

RBP1 plays a significant role in the retinoic acid signaling pathway affecting gene expression regulation by modulating retinoid concentrations. It interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) pathways influencing transcriptional activities related to development and differentiation. Connections between RBP1 and LRAT are important for the retinol esterification process integrating into broader retinoid metabolism and signaling pathways that govern cellular functions.

Dysregulation of RBP1 can contribute to conditions such as age-related macular degeneration (AMD) and metabolic syndrome. In AMD impaired retinol transport and metabolism lead to retinal damage and vision loss. In metabolic syndrome altered retinoid homeostasis involving RBP1 may exacerbate insulin resistance and obesity. Relationships with proteins like LRAT and retinaldehyde dehydrogenase (RALDH) highlight its involvement in disease mechanisms related to retinol dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoplasmic retinol-binding protein (PubMed : 22665496, PubMed : 26900151, PubMed : 28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed : 15632377, PubMed : 22665496).
See full target information RBP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 11:1479-1496 PubMed35958325

2022

Identification of a three-gene expression signature and construction of a prognostic nomogram predicting overall survival in lung adenocarcinoma based on TCGA and GEO databases.

Applications

Unspecified application

Species

Unspecified reactive species

Yuwei Zhou,Shenhu Gao,Rong Yang,Chengli Du,Yanli Wang,Yihe Wu
View all publications
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Product promise

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