Rabbit Polyclonal RET1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RBP1 aa 1 to C-terminus.
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Publications
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- Cellular signalling :1099502021WITHDRAWN: Circ_0000517 contributes to hepatocellular carcinoma progression by upregulating ARID4B via sponging miR-328-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongmei Zhao,Ya Sun,Jing Yang,Zhenfeng Zhu,Xin JiaPubMed 33582185
- Frontiers in cellular neuroscience 14:1232020Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica Sadler et. al.PubMed 32528249
- Scientific reports 10:38502020Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCéline Hoffmann et. al.PubMed 32123215
- Human molecular genetics :2019The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Berner et. al.PubMed 30986821
- The Journal of biological chemistry 292:15080-150932017The retinol-binding protein receptor STRA6 regulates diurnal insulin responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristy M Gliniak,J Mark Brown,Noa NoyPubMed 28733465
- Journal of ophthalmology 2015:9184732015iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RBP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09455
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Cytoplasmic retinol-binding protein (PubMed:22665496, PubMed:26900151, PubMed:28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed:15632377, PubMed:22665496).
Alternative names
CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I
Recommended products
Rabbit Polyclonal RET1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human RBP1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RBP1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09455
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Retinol binding protein 1 (RBP1) also known as cellular retinol-binding protein 1 (CRBP1) plays a critical role in the regulation and transportation of retinol a form of vitamin A within cells. RBP1 has a molecular mass of approximately 15 kDa and localizes predominantly in the cytoplasm. It is expressed in multiple tissues including the liver kidney and adipose tissue where it helps in regulating retinoid metabolism. The presence of RBP1 in these tissues is essential for maintaining physiological retinol levels.
- Biological function summary
RBP1 assists in the cellular uptake storage and metabolism of retinol facilitating its conversion into retinal and retinoic acid. Although it is not part of a well-defined complex RBP1 works closely with enzymes involved in retinol metabolism such as lecithin retinol acyltransferase (LRAT) and retinal dehydrogenases. This process is important for maintaining visual function immune response and cellular differentiation which all rely on proper retinol metabolism and activity.
- Pathways
RBP1 plays a significant role in the retinoic acid signaling pathway affecting gene expression regulation by modulating retinoid concentrations. It interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) pathways influencing transcriptional activities related to development and differentiation. Connections between RBP1 and LRAT are important for the retinol esterification process integrating into broader retinoid metabolism and signaling pathways that govern cellular functions.
- Associated diseases and disorders
Dysregulation of RBP1 can contribute to conditions such as age-related macular degeneration (AMD) and metabolic syndrome. In AMD impaired retinol transport and metabolism lead to retinal damage and vision loss. In metabolic syndrome altered retinoid homeostasis involving RBP1 may exacerbate insulin resistance and obesity. Relationships with proteins like LRAT and retinaldehyde dehydrogenase (RALDH) highlight its involvement in disease mechanisms related to retinol dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-RBP1 antibody (ab154881)
15% SDS PAGE
All lanes: Western blot - Anti-RBP1 antibody (ab154881) at 1/5000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Western blot - Anti-RBP1 antibody (ab154881)
12 % SDS-PAGE
All lanes: Western blot - Anti-RBP1 antibody (ab154881) at 1/5000 dilution
Lane 1: NIH3T3 whole cell lysate at 30 µg
Lane 2: C2C12 whole cell lysate at 30 µg
Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (ab154881)
Immunofluorescence analysis of methanol fixed H1299 cells, labeling RBP1 using ab154881 at 1/500 dilution. Lower image costained with Hoerchst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP1 antibody (ab154881)
Immunohistochemical analysis of paraffin embedded Human colon tissue, labeling RBP1 using ab154881 at 1/100 dilution.
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