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AB31106

Anti-RBP1 antibody

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(2 Publications)

Goat Polyclonal RET1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RBP1 aa 100 to C-terminus.

View Alternative Names

CRBP1, RBP1, Retinol-binding protein 1, Cellular retinol-binding protein, Cellular retinol-binding protein I, CRBP, CRBP-I

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (AB31106)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (AB31106)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Western blot - Anti-RBP1 antibody (AB31106)
  • WB

Unknown

Western blot - Anti-RBP1 antibody (AB31106)

All lanes:

Western blot - Anti-RBP1 antibody (ab31106) at 0.1 µg/mL

All lanes:

NIH 3T3 lysate in RIPA buffer at 35 µg

Predicted band size: 16 kDa

Observed band size: 16 kDa,18 kDa

true

Exposure time: 1hr

Western blot - Anti-RBP1 antibody (AB31106)
  • WB

Supplier Data

Western blot - Anti-RBP1 antibody (AB31106)

Lane 1:

Western blot - Anti-RBP1 antibody (ab31106) at 2 µg/mL

Lane 2:

Western blot - Anti-RBP1 antibody (ab31106) at 0.5 µg/mL

Lane 1:

NIH3T3 cell lysate at 35 µg

Lane 2:

U251 cell lysate at 35 µg

Predicted band size: 16 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RBP1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09455

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Ab31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Retinol binding protein 1 (RBP1) also known as cellular retinol-binding protein 1 (CRBP1) plays a critical role in the regulation and transportation of retinol a form of vitamin A within cells. RBP1 has a molecular mass of approximately 15 kDa and localizes predominantly in the cytoplasm. It is expressed in multiple tissues including the liver kidney and adipose tissue where it helps in regulating retinoid metabolism. The presence of RBP1 in these tissues is essential for maintaining physiological retinol levels.
Biological function summary

RBP1 assists in the cellular uptake storage and metabolism of retinol facilitating its conversion into retinal and retinoic acid. Although it is not part of a well-defined complex RBP1 works closely with enzymes involved in retinol metabolism such as lecithin retinol acyltransferase (LRAT) and retinal dehydrogenases. This process is important for maintaining visual function immune response and cellular differentiation which all rely on proper retinol metabolism and activity.

Pathways

RBP1 plays a significant role in the retinoic acid signaling pathway affecting gene expression regulation by modulating retinoid concentrations. It interacts with retinoic acid receptor (RAR) and retinoid X receptor (RXR) pathways influencing transcriptional activities related to development and differentiation. Connections between RBP1 and LRAT are important for the retinol esterification process integrating into broader retinoid metabolism and signaling pathways that govern cellular functions.

Dysregulation of RBP1 can contribute to conditions such as age-related macular degeneration (AMD) and metabolic syndrome. In AMD impaired retinol transport and metabolism lead to retinal damage and vision loss. In metabolic syndrome altered retinoid homeostasis involving RBP1 may exacerbate insulin resistance and obesity. Relationships with proteins like LRAT and retinaldehyde dehydrogenase (RALDH) highlight its involvement in disease mechanisms related to retinol dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoplasmic retinol-binding protein (PubMed : 22665496, PubMed : 26900151, PubMed : 28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed : 15632377, PubMed : 22665496).
See full target information RBP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32422974

2020

Proteomic Characterization of Colorectal Cancer Cells versus Normal-Derived Colon Mucosa Cells: Approaching Identification of Novel Diagnostic Protein Biomarkers in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maja Ludvigsen,Louise Thorlacius-Ussing,Henrik Vorum,Mary Pat Moyer,Mogens Tornby Stender,Ole Thorlacius-Ussing,Bent Honoré

Proteomics 10:3480-93 PubMed20815088

2010

Proteomic analysis of testis biopsies in men treated with transient scrotal hyperthermia reveals the potential targets for contraceptive development.

Applications

WB

Species

Human

Hui Zhu,Yugui Cui,Jin Xie,Ling Chen,Xiangxiang Chen,Xuejiang Guo,Yefei Zhu,Xinghai Wang,Jiansun Tong,Zuomin Zhou,Yue Jia,Yan-He Lue,Amiya Sinha Hikim,Christina Wang,Ronald S Swerdloff,Jiahao Sha
View all publications
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