AB180494

Anti-RBP2 antibody [EPR12334]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RET2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CRBP2, RBP2, Retinol-binding protein 2, Cellular retinol-binding protein II, CRBP-II

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (AB180494)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (AB180494)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling RBP2 with ab180494 at a 1/500 dilution. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (AB180494)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (AB180494)

Immunofluorescent analysis of 4% paraformaldehyde fixed SW480 cell line staining RBP2 using ab180494 at a 1/100 dilution. Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody at a dilution of 1/250. Counter stain : Dapi.

Supplier Data

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution

All lanes:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP2 antibody [EPR12334] (ab180494) at 1/5000 dilution

All lanes:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 16 kDa

false

Carrier free

Anti-RBP2 antibody [EPR12334] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12334

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP2 also known as KDM5A or Lysine Demethylase 5A functions as a histone demethylase enzyme specific for H3K4me3 and H3K4me2. With a mass of about 175 kDa it influences chromatin structure by removing methyl groups from histones which affects gene transcription. This protein expresses in various tissues especially in those with rapid cell division such as embryonic stem cells and certain cancer cell lines.

Biological function summary

RBP2 contributes to the regulation of gene expression by modifying chromatin accessibility. It forms part of transcriptional complexes that involve other chromatin-remodeling proteins. Through its demethylase activity RBP2 regulates the transcriptional response to different cellular signals and contributes to processes like cell cycle progression and differentiation.

Pathways

RBP2 plays a significant role in chromatin organization and transcriptional regulation pathways. RBP2 interacts closely with other chromatin-modifying proteins such as the Polycomb group of proteins impacting the Rb-E2F pathway involved in cell cycle control. This interaction influences whether cells proceed to division or undergo differentiation making it essential for maintaining proper cell growth and development.

RBP2 has connections to cancer and developmental disorders. Its abnormal expression often associates with various cancers including leukemia and breast cancer contributing to uncontrolled cell proliferation and tumor growth. In these contexts RBP2 may interact with proteins like pRB and E2F which are important in cell cycle regulation highlighting its importance in cancer biology. Additionally mutations in RBP2 can result in developmental abnormalities further highlighting its critical role in normal cellular processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Intracellular transport of retinol.

See full target information RBP2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Current topics in developmental biology 161:89-111 PubMed39870440

2025

Retinoids and retinoid-binding proteins: Unexpected roles in metabolic disease.

Applications

Unspecified application

Species

Unspecified reactive species

William S Blaner,Jisun Paik,Pierre-Jacques Brun,Marcin Golczak

Cell stem cell 29:101-115.e10 PubMed34582804

2021

SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gu,Hua Wang,Xiaofeng Huang,Judith Kraiczy,Pratik N P Singh,Charles Ng,Sezin Dagdeviren,Sean Houghton,Oscar Pellon-Cardenas,Ying Lan,Yaohui Nie,Jiaoyue Zhang,Kushal K Banerjee,Emily J Onufer,Brad W Warner,Jason Spence,Ellen Scherl,Shahin Rafii,Richard T Lee,Michael P Verzi,David Redmond,Randy Longman,Kristian Helin,Ramesh A Shivdasani,Qiao Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBP2 antibody [EPR12334]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP2 antibody [EPR12334] (AB180494)

Immunocytochemistry/ Immunofluorescence - Anti-RBP2 antibody [EPR12334] (AB180494)

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Western blot - Anti-RBP2 antibody [EPR12334] (AB180494)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise