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AB222192

Anti-RBP3/IRBP antibody

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(1 Publication)

Rabbit Polyclonal RBP3/IRBP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RBP3 aa 900-1050.

View Alternative Names

Retinol-binding protein 3, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, IRBP, RBP3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP3/IRBP antibody (AB222192)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP3/IRBP antibody (AB222192)

Immunohistochemistry analysis of human eye tissue labeling RBP3 with ab222192 at 1 : 1000 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RBP3 aa 900-1050. The exact immunogen used to generate this antibody is proprietary information.

P10745

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Alternative Products

Primary Antibodies

AB233190

Anti-RBP3/IRBP antibody

Western blot - Anti-RBP3/IRBP antibody (AB233190)

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Complementary Products

Assay Kits

AB190535

Mouse Retinol Binding Protein ELISA Kit (RBP)

Sandwich ELISA - Mouse Retinol Binding Protein ELISA Kit (RBP) (AB190535)

  • 0
  • 0
Primary Antibodies

AB307684

Anti-RBP1 antibody [42/RBP] - BSA and Azide free

Recombinant

Western blot - Anti-RBP1 antibody [42/RBP] - BSA and Azide free (AB307684)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB215617

Recombinant Human RBP3/IRBP protein (His tag)

SDS-PAGE - Recombinant Human RBP3/IRBP protein (His tag) (AB215617)

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Primary Antibodies

AB234187

Anti-DR3/LARD antibody [JD3]

Flow Cytometry - Anti-DR3/LARD antibody [JD3] (AB234187)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RBP3 protein also known as Interphotoreceptor Retinoid-Binding Protein (IRBP) plays a significant mechanical role in the eye. This protein is characterized by a mass of approximately 135 kDa and is primarily expressed in the retina and vitreous body. RBP3 is involved in the transport and protection of retinoids between the photoreceptor cells and the retinal pigment epithelium. It facilitates the movement of molecules necessary for the visual cycle ensuring efficient communication between different cellular components of the eye.
Biological function summary

RBP3 is integral in maintaining retinal health aiding the regeneration of visual pigments and the function of photosensitive cells. It does not appear to be part of a larger complex but operates independently within the extracellular space between the retina cells. The protein binds retinoids including retinol and retinaldehyde which are critical for converting light into visual signals. This function supports the overall visual transduction pathway in vertebrates.

Pathways

RBP3 is an important player in the visual cycle pathway maintaining normal vision by aiding in the synthesis and recycling of visual pigments. It works closely with proteins like opsin which is involved in the photoactivation required for vision. Furthermore RBP3 interacts within the retinoid cycle in the retina ensuring that retinoids are efficiently recycled and available for phototransduction.

RBP3 has connections with retinitis pigmentosa and age-related macular degeneration both of which are severe degenerative disorders of the retina. Mutations or deficiencies in RBP3 can disrupt retinoid transport leading to impaired vision or vision loss. This protein may be linked to other visual cycle proteins such as RPE65 which also play roles in the development of these diseases. Understanding RBP3's function and interaction with other proteins helps in developing therapeutic strategies for these retinal conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.
See full target information RBP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 219: PubMed31881080

2019

Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Poser,Renaud Caous,Ulrike Gruneberg,Francis A Barr
View all publications
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Product promise

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