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AB154914

Anti-RBP4 antibody

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(3 Publications)

Rabbit Polyclonal RBP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RBP4 aa 1-200.

View Alternative Names

PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody (AB154914)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody (AB154914)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling RBP4 with ab154914 at 1/200 dilution. The image in the lower panel is merged with a DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody (AB154914)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody (AB154914)

Immunohistochemical analysis of paraffin-embedded OECM1 xenograft tissue labeling RBP4 with ab154914 at 1/500 dilution.

Western blot - Anti-RBP4 antibody (AB154914)
  • WB

Unknown

Western blot - Anti-RBP4 antibody (AB154914)

12% SDS PAGE

All lanes:

Western blot - Anti-RBP4 antibody (ab154914) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RBP4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P02753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Tris-EDTA buffer (pH 8.0) can also be used.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed : 5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed : 22665496).
See full target information RBP4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 10:2083-2094 PubMed34584879

2021

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Gedong Ming,Wanliang Guo,Yuan Cheng,Jian Wang

Molecular pharmaceutics 16:3237-3252 PubMed31136717

2019

Hydrophilic Small Molecules That Harness Transthyretin To Enhance the Safety and Efficacy of Targeted Chemotherapeutic Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Arindom Pal,Wabel Albusairi,Fang Liu,Md Tariqul Haque Tuhin,Mark Miller,Dengpan Liang,Hyun Joo,Toufiq Ul Amin,Elizabeth A Wilson,Jesika S Faridi,Miki Park,Mamoun M Alhamadsheh

Nature chemical biology 11:793-8 PubMed26344696

2015

A biomimetic approach for enhancing the in vivo half-life of peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Sravan C Penchala,Mark R Miller,Arindom Pal,Jin Dong,Nikhil R Madadi,Jinghang Xie,Hyun Joo,Jerry Tsai,Patrick Batoon,Vyacheslav Samoshin,Andreas Franz,Trever Cox,Jesse Miles,William K Chan,Miki S Park,Mamoun M Alhamadsheh
View all publications
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Product promise

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