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AB109193

Anti-RBP4 antibody [EP3657]

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(18 Publications)

Rabbit Recombinant Monoclonal RBP4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 18 publications.

View Alternative Names

PRO2222, RBP4, Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)

Immunofluorescent analysis of HeLa cells labelling RBP4 with un-purified ab109193 at a dilution of 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EP3657] (AB109193)

Immunocytochemistry/immunofluorescence staining of 100% methanol fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab109193 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Supplier Data

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 2:

Human fetal kidney at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Unknown

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/1000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Supplier Data

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

All lanes:

Mouse serum at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)
  • WB

Unknown

Western blot - Anti-RBP4 antibody [EP3657] (AB109193)

Blocking and diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution

All lanes:

Rat serum at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

  • Carrier free

    Anti-RBP4 antibody [EP3657] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3657

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.18% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
Biological function summary

RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.

Pathways

RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.

RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed : 5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed : 22665496).
See full target information RBP4
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 20:77 PubMed40442809

2025

The effect and mechanism of Germacrone in ameliorating alcoholic fatty liver by inhibiting Nrf2/Rbp4.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Xiao,Jiamin Fang,Qinpo Huang,Guolin He,Xia Ou,Yang De,Shuhua Gui,Yun Zhang,Maoci Wang,Yiyuan Zhong,Dawa Zeren,Yongling Long,Changhui Liu,Tianqin Xiong

Genes 16: PubMed40004479

2025

Retinol-Binding Protein 4 as a Biomarker in Cancer: Insights from a Pan-Cancer Analysis of Expression, Immune Infiltration, and Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhao,Yaxin Liu,Lingqin Zhou,Yi Liu

Journal of cachexia, sarcopenia and muscle 15:1601-1615 PubMed39031684

2024

RBP4 promotes denervation-induced muscle atrophy through STRA6-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Zhen Zhang,Jia-Wen Li,Jin-Shui Xu,Zheng-Kai Shen,Yu-Shuang Lin,Can Zhao,Xiang Lu,Yun-Feng Rui,Wei Gao

Central-European journal of immunology 49:366-382 PubMed39944260

2024

LncRNA RMRP promotes chondrocyte injury by regulating the FOXC1/RBP4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Gang Zhou,Te Chen,Qiao Lin,Qiupeng Yang

Microscopy research and technique 86:223-231 PubMed36354743

2022

Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Xu,Shi Bai,Haijian Wu,Marong Fang

Molecular reproduction and development 89:423-430 PubMed35818817

2022

miR-24-3p regulation of retinol binding protein 4 in trophoblast biofunction and preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Li,Xiaoli Ru,Shuzhen Wang,Guangming Cao

Bone research 10:38 PubMed35477573

2022

A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yishan Chen,Yeke Yu,Ya Wen,Juan Chen,Junxin Lin,Zixuan Sheng,Wenyan Zhou,Heng Sun,Chengrui An,Jiansong Chen,Weiliang Wu,Chong Teng,Wei Wei,Hongwei Ouyang

Journal of the American Heart Association 10:e022011 PubMed34726071

2021

Retinol-Binding Protein 4 Promotes Cardiac Injury After Myocardial Infarction Via Inducing Cardiomyocyte Pyroptosis Through an Interaction With NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Zhen Zhang,Xi-Yu Shen,Man Wang,Li Wang,Hui-Xian Sun,Xiu-Zhen Li,Jing-Jing Huang,Xiao-Qing Li,Cheng Wu,Can Zhao,Jia-Li Liu,Xiang Lu,Wei Gao

Nature communications 12:2783 PubMed33986294

2021

Alteration of circadian machinery in monocytes underlies chronic kidney disease-associated cardiac inflammation and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Yoshida,Naoya Matsunaga,Takaharu Nakao,Kengo Hamamura,Hideaki Kondo,Tomomi Ide,Hiroyuki Tsutsui,Akito Tsuruta,Masayuki Kurogi,Michio Nakaya,Hitoshi Kurose,Satoru Koyanagi,Shigehiro Ohdo

American journal of physiology. Endocrinology and metabolism 320:E306-E315 PubMed33284085

2020

Retinol-binding protein 4 mRNA translation in hepatocytes is enhanced by activation of mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Jaclyn E Welles,Allyson L Toro,Siddharth Sunilkumar,Shaunaci A Stevens,Carson J Purnell,Scot R Kimball,Michael D Dennis
View all publications
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