Rabbit Recombinant Monoclonal RBP4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--western-blot-img126074.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110859.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137)")
![OI-RD Scanning - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--oi-rd-scanning-img155573.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (AB226137)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Retinol-binding protein that mediates retinol transport in blood plasma (PubMed:5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed:22665496).
Alternative names
Retinol-binding protein 4, Plasma retinol-binding protein, PRBP, RBP, RBP4, PRO2222
Recommended products
Rabbit Recombinant Monoclonal RBP4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR5878
- Purification technique
Affinity purification Protein A
- Dissociation constant
1.69 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226137 is the carrier-free version of Anti-RBP4 antibody [EPR5878] ab133530.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBP4 or retinol-binding protein 4 is a carrier protein that is essential for the transport of retinol or vitamin A in the bloodstream. Also known by the names RBP or plasma retinol-binding protein this protein has a molecular weight of approximately 21 kDa. RBP4 is mainly expressed in the liver and secreted into the blood where it binds to retinol and protects it from oxidation. The structure of RBP4 allows it to fit into binding pockets facilitating the transport of retinol to peripheral tissues.
- Biological function summary
RBP4 plays a role as a transporter of vitamin A from the liver to tissues where it is needed. It forms complexes with transthyretin in the blood which prevents kidney filtration and subsequent loss of retinol. This function ensures the proper delivery and availability of retinol essential for vision cellular differentiation and immune system function. The stability of the retinol-RBP4 complex is important to maintaining sufficient vitamin A levels in the body.
- Pathways
RBP4 is significant in the vitamin A metabolism pathway and affects the retinoid signaling pathways. These pathways are critical for processes such as vision and skin health. RBP4 interacts with proteins like lecithin retinol acyltransferase (LRAT) in these pathways which are responsible for esterifying retinol into retinyl esters a storage form of vitamin A. These interactions ensure the efficient transport and storage of vitamin A and that it is accessible when needed by different tissues.
- Associated diseases and disorders
RBP4 is linked to the development of insulin resistance and type 2 diabetes. Elevated RBP4 levels correlate with these conditions and highlight its role in metabolic processes. Studies also show a connection between altered RBP4 levels and obesity with insulin resistance often occurring in conjunction with obesity. The protein is implicated in these metabolic disorders through its interactions with insulin-regulating proteins which influence glucose homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--western-blot-img126074.jpg "Western blot - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)")
Western blot - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)
This data was developed using Anti-RBP4 antibody [EPR5878] ab133530, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-RBP4 antibody [EPR5878] (Anti-RBP4 antibody [EPR5878] ab133530) at 1/1000 dilution
Lane 1: Human plasma lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
Lane 3: HepG2 lysate at 10 µg
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110859.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)
This data was developed using Anti-RBP4 antibody [EPR5878] ab133530, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling RBP4 with Anti-RBP4 antibody [EPR5878] ab133530 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137), expandable thumbnail](https://content.abcam.com/products/images/rbp4-antibody-epr5878-bsa-and-azide-free-ab226137--oi-rd-scanning-img155573.jpg "OI-RD Scanning - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)")
OI-RD Scanning - Anti-RBP4 antibody [EPR5878] - BSA and Azide free (ab226137)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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