JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB217683

Anti-RBPJK antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal RBPJK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RBPJ aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPJK antibody (AB217683)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPJK antibody (AB217683)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue, labeling RBPJK with ab217683 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RBPJ aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q06330

style
left-column

Alternative Products

Primary Antibodies

AB25949

Anti-RBPJK antibody

Immunoprecipitation - Anti-RBPJK antibody (AB25949)

  • 4
  • 9
Primary Antibodies

AB252858

Anti-RBPJK antibody [7A11]

Recombinant

Western blot - Anti-RBPJK antibody [7A11] (AB252858)

  • 0
  • 0
Primary Antibodies

AB180588

Anti-RBPJK antibody [EPR13479]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] (AB180588)

  • 4
  • 3
Primary Antibodies

AB255852

Anti-RBPJK antibody [7A11] - BSA and Azide free

Recombinant

Western blot - Anti-RBPJK antibody [7A11] - BSA and Azide free (AB255852)

  • 0
  • 0
Primary Antibodies

AB240227

Anti-RBPJK antibody [EPR13479] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB52627

Anti-Notch1 antibody [EP1238Y]

RabMAb|Recombinant|KO Validated|20ul selling size|Advanced Validation

Western blot - Anti-Notch1 antibody [EP1238Y] (AB52627)

  • 5
  • 18
Primary Antibodies

AB108937

Anti-Hes1 antibody [EPR4226]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody [EPR4226] (AB108937)

  • 4
  • 15
Primary Antibodies

AB23426

Anti-NOTCH3 antibody

BOND RX™ Validated

Western blot - Anti-NOTCH3 antibody (AB23426)

  • 4
  • 22
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBPJK also known as CSL or CBF1 is a transcriptional regulator that plays an important role in the Notch signaling pathway. It has a molecular mass of approximately 60 kDa. RBPJK expression occurs in multiple tissues including the thymus brain and heart. This protein operates mechanically by binding to DNA and mediating the transcriptional activity of Notch target genes.
Biological function summary

The protein functions as a central component of the Notch signaling complex. It interacts with other proteins to regulate the expression of genes that guide cell fate decisions. RBPJK forms complexes with Notch intracellular domain and Mastermind-like (MAML) co-activators and it can switch between repressor or activator of transcription depending on its binding partners. This regulation ensures proper development and tissue homeostasis.

Pathways

RBPJK influences processes that are critical in cell differentiation and proliferation. It resides within the Notch signaling pathway where it is essential for transmitting Notch receptor signals into the cell nucleus. RBPJK associates with proteins such as Notch1 and MAML1 facilitating a cascade of events important for determining cellular outcomes during developmental processes and maintaining stem cell populations.

Dysregulation of RBPJK has connections to cancer and Alzheimer’s disease. In cancer aberrant Notch signaling often involving RBPJK leads to unchecked cell proliferation and survival making it a target for drug development. Alzheimer's disease studies reveal that RBPJK may influence the processing of amyloid precursor protein with possible links to abnormal protein aggregation. Understanding interactions with proteins like NICD and APP is important for unraveling its role in these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA (PubMed : 21991380). Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (PubMed : 23303788). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
See full target information RBPJ

Alternative Names

IGKJRB, IGKJRB1, RBPJK, RBPSUH, RBPJ, Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, Renal carcinoma antigen NY-REN-30, RBP-J, RBP-JK
style
left-column

Publications (1)

Recent publications for all applications. Explore the full listand refine your search

PLoS pathogens 21:e1013029 PubMed40825050

2025

LANA-dependent transcription-replication conflicts and R-loops at the terminal repeats (TR) correlate with KSHV episome maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Asim Asghar,Olga Vladimirova,Asher Sobotka,James Hayden,Jayamanna Wickramasinghe,Jayaraju Dheekollu,Moeko Minakuchi,Leena Yoon,Maureen E Murphy,Kazuko Nishikura,Paul M Lieberman
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com