Rabbit Recombinant Monoclonal RBPJK antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--immunocytochemistry-immunofluorescence-img44180.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)")
![Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--flow-cytometry-intracellular-img27529.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)")
![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--immunocytochemistry-immunofluorescence-img93946.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Not recommended |
| Mouse | Expected | Predicted | Predicted | Not recommended | Not recommended |
| Rat | Expected | Predicted | Predicted | Not recommended | Not recommended |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA (PubMed:21991380). Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (PubMed:23303788). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
Alternative names
IGKJRB, IGKJRB1, RBPJK, RBPSUH, RBPJ, Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, Renal carcinoma antigen NY-REN-30, RBP-J, RBP-JK
Recommended products
Rabbit Recombinant Monoclonal RBPJK antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13479
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240227 is the carrier-free version of Anti-RBPJK antibody [EPR13479] ab180588.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBPJK also known as CSL or CBF1 is a transcriptional regulator that plays an important role in the Notch signaling pathway. It has a molecular mass of approximately 60 kDa. RBPJK expression occurs in multiple tissues including the thymus brain and heart. This protein operates mechanically by binding to DNA and mediating the transcriptional activity of Notch target genes.
- Biological function summary
The protein functions as a central component of the Notch signaling complex. It interacts with other proteins to regulate the expression of genes that guide cell fate decisions. RBPJK forms complexes with Notch intracellular domain and Mastermind-like (MAML) co-activators and it can switch between repressor or activator of transcription depending on its binding partners. This regulation ensures proper development and tissue homeostasis.
- Pathways
RBPJK influences processes that are critical in cell differentiation and proliferation. It resides within the Notch signaling pathway where it is essential for transmitting Notch receptor signals into the cell nucleus. RBPJK associates with proteins such as Notch1 and MAML1 facilitating a cascade of events important for determining cellular outcomes during developmental processes and maintaining stem cell populations.
- Associated diseases and disorders
Dysregulation of RBPJK has connections to cancer and Alzheimer’s disease. In cancer aberrant Notch signaling often involving RBPJK leads to unchecked cell proliferation and survival making it a target for drug development. Alzheimer's disease studies reveal that RBPJK may influence the processing of amyloid precursor protein with possible links to abnormal protein aggregation. Understanding interactions with proteins like NICD and APP is important for unraveling its role in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--immunocytochemistry-immunofluorescence-img44180.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)")
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)
This image was made using Anti-RBPJK antibody [EPR13479] ab180588 which is the same antibody as ab240227 with BSA and Azide
Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified Anti-RBPJK antibody [EPR13479] ab180588 at 1:100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--flow-cytometry-intracellular-img27529.jpg "Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)")
Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)
This image was made using Anti-RBPJK antibody [EPR13479] ab180588 which is the same antibody as ab240227 with BSA and Azide Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RBPJK with Purified Anti-RBPJK antibody [EPR13479] ab180588 at 1/100 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227), expandable thumbnail](https://content.abcam.com/products/images/rbpjk-antibody-epr13479-bsa-and-azide-free-ab240227--immunocytochemistry-immunofluorescence-img93946.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)")
Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (ab240227)
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling RBPJK with unpurified Anti-RBPJK antibody [EPR13479] ab180588 at 1/100 (green). Dapi shown in blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-RBPJK antibody [EPR13479] ab180588).
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