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AB240227

Anti-RBPJK antibody [EPR13479] - BSA and Azide free

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Rabbit Recombinant Monoclonal RBPJK antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

IGKJRB, IGKJRB1, RBPJK, RBPSUH, RBPJ, Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, Renal carcinoma antigen NY-REN-30, RBP-J, RBP-JK

3 Images
Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)

This data was developed using ab180588, the same antibody clone in a different buffer formulation.

Flow cytometry overlay histogram of 4% Paraformaldehyde MG 63 (human osteosarcoma fibroblast) cells permeabilised with 90% methanol labelling RBPJK with ab180588 at 1/50 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) , at 1/5000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)

This data was developed using ab180588, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of MG 63 (human osteosarcoma fibroblast) cells labeling RBPJK with Purified ab180588 at 1 : 50 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1 : 200 dilution. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) was used as the secondary antibody at 1 : 1000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control.

Confocal image showing mainly nuclear staining in MG-63 cell line(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Western blot - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)
  • WB

Lab

Western blot - Anti-RBPJK antibody [EPR13479] - BSA and Azide free (AB240227)

This data was developed using ab180588, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RBPJK antibody [EPR13479] (<a href='/en-us/products/primary-antibodies/rbpjk-antibody-epr13479-ab180588'>ab180588</a>)

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa

false

Exposure time: 48s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13479

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab240227 is the carrier-free version of ab180588.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBPJK also known as CSL or CBF1 is a transcriptional regulator that plays an important role in the Notch signaling pathway. It has a molecular mass of approximately 60 kDa. RBPJK expression occurs in multiple tissues including the thymus brain and heart. This protein operates mechanically by binding to DNA and mediating the transcriptional activity of Notch target genes.
Biological function summary

The protein functions as a central component of the Notch signaling complex. It interacts with other proteins to regulate the expression of genes that guide cell fate decisions. RBPJK forms complexes with Notch intracellular domain and Mastermind-like (MAML) co-activators and it can switch between repressor or activator of transcription depending on its binding partners. This regulation ensures proper development and tissue homeostasis.

Pathways

RBPJK influences processes that are critical in cell differentiation and proliferation. It resides within the Notch signaling pathway where it is essential for transmitting Notch receptor signals into the cell nucleus. RBPJK associates with proteins such as Notch1 and MAML1 facilitating a cascade of events important for determining cellular outcomes during developmental processes and maintaining stem cell populations.

Dysregulation of RBPJK has connections to cancer and Alzheimer’s disease. In cancer aberrant Notch signaling often involving RBPJK leads to unchecked cell proliferation and survival making it a target for drug development. Alzheimer's disease studies reveal that RBPJK may influence the processing of amyloid precursor protein with possible links to abnormal protein aggregation. Understanding interactions with proteins like NICD and APP is important for unraveling its role in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA (PubMed : 21991380). Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (PubMed : 23303788). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).
See full target information RBPJ
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com