Anti-RBPMS antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (AB152101)

Paraffin-embedded rat colon tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (AB152101)

Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labelling RBPMS protein at cytoplasm and nucleus using ab152101 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 dilution (Red).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (AB152101)

Immunofluorescence analysis of 4% paraformaldehyde fixed A431 cells labelling RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (AB152101)

Paraffin-embedded rat lymph node tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/2000 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (AB152101)

Paraffin-embedded mouse lymph node tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (AB152101)

Paraffin-embedded rat kidney tissue stained for RBPMS protein with ab152101 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (AB152101)

Paraffin-embedded mouse retina tissue stained for RBPMS protein with ab152101 at a 1/250 dilution in immunohistochemical analysis.
Red : beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1/500 dilution.
Blue : DAPI.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-RBPMS antibody (AB152101)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-RBPMS antibody (ab152101) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

• WB

Supplier Data

Western blot - Anti-RBPMS antibody (AB152101)

Samples were separated by SDS-PAGE.

All lanes:

Western blot - Anti-RBPMS antibody (ab152101) at 1/500 dilution

Lane 1:

Non-transfected HCT-116 cells

Lane 2:

Transfected HCT-116 cells

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

• WB

Lab

Western blot - Anti-RBPMS antibody (AB152101)

Lanes 1 - 4 : Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

Lanes 1 - 4 : Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab152101 was shown to react with RBPMS in wild-type HeLa cells in western blot. The bands observed in RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631) below 30 kDa may represent truncated forms and cleaved fragments. This has not been investigated further and the functional properties of the gene product have not been determined. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab152101 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-RBPMS antibody (<a href='/en-us/products/primary-antibodies/rbpms-antibody-ab194213'>ab194213</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-RBPMS antibody (ab152101) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RBPMS Knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RBPMS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbpms-knockout-hela-cell-line-ab264697'>ab264697</a>)

Lane 3:

RT-4 cell lysate at 20 µg

Lane 4:

U-251 MG cell lysate at 20 µg

Predicted band size: 22 kDa

Observed band size: 30 kDa

false

• WB

Supplier Data

Western blot - Anti-RBPMS antibody (AB152101)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-RBPMS antibody (ab152101) at 1/1000 dilution

Lane 1:

Non-transfected 293T cells at 30 µg

Lane 2:

Transfected 293T cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false