Anti-RBPMS antibody (ab152101) is a rabbit polyclonal antibody detecting RBPMS in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 20 publications
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- Investigative ophthalmology & visual science 64:282023Resveratrol Alleviates Retinal Ischemia-Reperfusion Injury by Inhibiting the NLRP3/Gasdermin D/Caspase-1/Interleukin-1β Pyroptosis Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Xie,Qian Ying,Hongdou Luo,Mengqi Qin,Yulian Pang,Haijian Hu,Jing Zhong,Yuning Song,Ziqiao Zhang,Xu ZhangPubMed 38133508
- Cells 12:2023Mitophagy in Astrocytes Is Required for the Health of Optic Nerve.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeysam Yazdankhah,Sayan Ghosh,Haitao Liu,Stacey Hose,J Samuel Zigler,Debasish SinhaPubMed 37887340
- Frontiers in aging neuroscience 15:12181932023Altered circadian behavior and light sensing in mouse models of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThaddeus K Weigel,Cherry L Guo,Ali D Güler,Heather A FerrisPubMed 37409006
- Acta neuropathologica communications 11:832023Retina-to-brain spreading of α-synuclein after intravitreal injection of preformed fibrils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDayana Pérez-Acuña,Ka Hyun Rhee,Soo Jean Shin,Jeeyun Ahn,Jee-Young Lee,Seung-Jae LeePubMed 37210559
- Molecular vision 29:39-572023Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney Linne,Khine Yin Mon,Shane D'Souza,Heonuk Jeong,Xiaoyan Jiang,Dillon M Brown,Kevin Zhang,Shruti Vemaraju,Kazuo Tsubota,Toshihide Kurihara,Machelle T Pardue,Richard A LangPubMed 37287644
- Redox biology 62:1026872023Pathologically high intraocular pressure induces mitochondrial dysfunction through Drp1 and leads to retinal ganglion cell PANoptosis in glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhou Zeng,Mengling You,Cong Fan,Rong Rong,Haibo Li,Xiaobo XiaPubMed 36989574
- Annals of translational medicine 11:32023Lipocalin 2 induces visual impairment by promoting ferroptosis in retinal ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingfang Mei,Jinwen Wu,Keling Wu,Minglei Zhao,Jingyi Luo,Xinqi Liu,Bizhi Shang,Wenchang Xu,Zeqiu Yang,Yuhua Lai,Chujun Liu,Haijun Gong,Xinbo Gao,Yehong Zhuo,Mingkai Lin,Ling ZhaoPubMed 36760251
- Redox biology 58:1025412022Inhibition of ferroptosis promotes retina ganglion cell survival in experimental optic neuropathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiao Guo,Yanfang Zhu,Ying Shi,Xiangda Meng,Xue Dong,Haokun Zhang,Xiaohong Wang,Mei Du,Hua YanPubMed 36413918
- Microscopy research and technique 86:223-2312022Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiwei Xu,Shi Bai,Haijian Wu,Marong FangPubMed 36354743
- EBioMedicine 85:1043142022Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley A Rowe,Xin Chen,Emily R Nettesheim,Yacine Issioui,Thomas Dong,Yuhui Hu,Souad Messahel,Saima N Kayani,Steven J Gray,Katherine J WertPubMed 36374771
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RBPMS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q93062
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Isoform A. RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS) (PubMed:24860013, PubMed:26347403). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets (By similarity). Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state (By similarity). Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats (PubMed:24860013, PubMed:26347403). Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes (By similarity). Isoform C. Acts as a regulator of pre-mRNA alternative splicing (AS) (By similarity). Binds mRNA (PubMed:17099224). Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA (By similarity). Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins (PubMed:17099224).
Alternative names
HERMES, RBPMS, RNA-binding protein with multiple splicing, RBP-MS, Heart and RRM expressed sequence, Hermes
Recommended products
Anti-RBPMS antibody (ab152101) is a rabbit polyclonal antibody detecting RBPMS in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RBPMS aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q93062
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-RBPMS antibody (ab152101) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of RBPMS?
Anti-RBPMS (ab152101) specifically detects a band for RBPMS (UniProt: Q93062) at a molecular weight of 24kDa.
Trusted by the scientific community
Anti-RBPMS (ab152101) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.
- Biological function summary
RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.
- Pathways
RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.
- Associated diseases and disorders
Mutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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12 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Paraffin-embedded rat colon tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western blot - Anti-RBPMS antibody (ab152101)
Lanes 1 - 4: Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.Lanes 1 - 4: Merged signal (red and green). Green - ab152101 observed at 30 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab152101 was shown to react with RBPMS in wild-type HeLa cells in western blot. The bands observed in RBPMS knockout cell line Human RBPMS knockout HeLa cell line ab264697 (RBPMS knockout cell lysate Human RBPMS knockout HeLa cell lysate ab258631) below 30 kDa may represent truncated forms and cleaved fragments. This has not been investigated further and the functional properties of the gene product have not been determined. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab152101 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 4: Western blot - Anti-RBPMS antibody (Anti-RBPMS antibody ab194213) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-RBPMS antibody (ab152101) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RBPMS Knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human RBPMS knockout HeLa cell line (Human RBPMS knockout HeLa cell line ab264697)
Lane 3: RT-4 cell lysate at 20 µg
Lane 4: U-251 MG cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling RBPMS with ab152101 at 1/250 dilution.
Western blot - Anti-RBPMS antibody (ab152101)
12% SDS PAGE
All lanes: Western blot - Anti-RBPMS antibody (ab152101) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 22 kDa, 28 kDa
Western blot - Anti-RBPMS antibody (ab152101)
12% SDS PAGE
All lanes: Western blot - Anti-RBPMS antibody (ab152101) at 1/1000 dilution
All lanes: Mouse heart whole cell lysate at 50 µg
Predicted band size: 22 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (ab152101)
Immunofluorescent analysis of methanol-fixed A549 cells labeling RBPMS with ab152101 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Paraffin-embedded mouse retina tissue stained for RBPMS protein with ab152101 at a 1/250 dilution in immunohistochemical analysis.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1/500 dilution.
Blue: DAPI.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (ab152101)
Immunofluorescence analysis of 4% paraformaldehyde fixed A431 cells labelling RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution at RT for 15 min (Green). The nuclear counter stain is DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Paraffin-embedded mouse lymph node tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Paraffin-embedded rat lymph node tissue stained for RBPMS protein at cytoplasm and nucleus with ab152101 at a 1/2000 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody (ab152101)
Paraffin-embedded rat kidney tissue stained for RBPMS protein with ab152101 at a 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence - Anti-RBPMS antibody (ab152101)
Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labelling RBPMS protein at cytoplasm and nucleus using ab152101 at a 1/500 dilution at RT for 15 min (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1/1000 dilution (Red).
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