Rabbit Polyclonal RBPMS antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 31 publications.
Images
Publications
- International journal of molecular sciences 24:2023The PKG Inhibitor CN238 Affords Functional Protection of Photoreceptors and Ganglion Cells against Retinal Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArianna Tolone,Wadood Haq,Alexandra Fachinger,Akanksha Roy,Sandeep Kesh,Andreas Rentsch,Sophie Wucherpfennig,Yu Zhu,John Groten,Frank Schwede,Tushar Tomar,Friedrich W Herberg,Vasilica Nache,François Paquet-DurandPubMed 37894958
- iScience 26:1077802023Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargaret E Maes,Gloria Colombo,Florianne E Schoot Uiterkamp,Felix Sternberg,Alessandro Venturino,Elena E Pohl,Sandra SiegertPubMed 37731609
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Tested |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins (PubMed:26347403). Binds to poly(A) RNA (PubMed:17099224, PubMed:26347403).
Alternative names
RNA-binding protein with multiple splicing, RBP-MS, Heart and RRM expressed sequence, Hermes, RBPMS, HERMES
Recommended products
Rabbit Polyclonal RBPMS antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 31 publications.
Alternative names
RNA-binding protein with multiple splicing, RBP-MS, Heart and RRM expressed sequence, Hermes, RBPMS, HERMES
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
RBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.
- Biological function summary
RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.
- Pathways
RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.
- Associated diseases and disorders
Mutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-RBPMS antibody (ab194213)
Lanes 1 - 4: Merged signal (red and green). Green - ab194213 observed at 30 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab194213 was shown to react with RBPMS in wild-type HeLa cells in western blot. Here no band was observed in the RBPMS knockout cell line Human RBPMS knockout HeLa cell line ab264697 (RBPMS knockout cell lysate Human RBPMS knockout HeLa cell lysate ab258631), but other antibodies have shown bands in this lysate below 30 kDa that may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab194213 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 4: Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution
Lanes 1 - 4: Western blot - Anti-RBPMS antibody (Anti-RBPMS antibody ab152101) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: RBPMS Knockout HeLa cell lysate at 20 µg
Lane 3: RT-4 cell lysate at 20 µg
Lane 4: U-251 MG cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa
Western blot - Anti-RBPMS antibody (ab194213)
All lanes: Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution
All lanes: Rat heart lysate
Predicted band size: 22 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com