Anti-RBPMS antibody

• WB

Lab

Western blot - Anti-RBPMS antibody (AB194213)

Lanes 1 - 4 : Merged signal (red and green). Green - ab194213 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab194213 was shown to react with RBPMS in wild-type HeLa cells in western blot. Here no band was observed in the RBPMS knockout cell line ab264697 (RBPMS knockout cell lysate ab258631), but other antibodies have shown bands in this lysate below 30 kDa that may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab194213 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

Lanes 1 - 4:

Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-RBPMS antibody (<a href='/en-us/products/primary-antibodies/rbpms-antibody-ab152101'>ab152101</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RBPMS Knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RBPMS knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rbpms-knockout-hela-cell-line-ab264697'>ab264697</a>)

Lane 3:

RT-4 cell lysate at 20 µg

Lane 4:

U-251 MG cell lysate at 20 µg

Predicted band size: 22 kDa

Observed band size: 30 kDa

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• WB

Supplier Data

Western blot - Anti-RBPMS antibody (AB194213)

All lanes:

Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution

All lanes:

Rat heart lysate

Predicted band size: 22 kDa

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