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Rabbit Polyclonal RBPMS antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 31 publications.


Images

Western blot - Anti-RBPMS antibody (AB194213), expandable thumbnail
  • Western blot - Anti-RBPMS antibody (AB194213), expandable thumbnail

Publications

  • International journal of molecular sciences 24:2023
    The PKG Inhibitor CN238 Affords Functional Protection of Photoreceptors and Ganglion Cells against Retinal Degeneration.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Arianna Tolone,Wadood Haq,Alexandra Fachinger,Akanksha Roy,Sandeep Kesh,Andreas Rentsch,Sophie Wucherpfennig,Yu Zhu,John Groten,Frank Schwede,Tushar Tomar,Friedrich W Herberg,Vasilica Nache,François Paquet-Durand
    PubMed 37894958
  • iScience 26:1077802023
    Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Margaret E Maes,Gloria Colombo,Florianne E Schoot Uiterkamp,Felix Sternberg,Alessandro Venturino,Elena E Pohl,Sandra Siegert
    PubMed 37731609

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.03% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Rat
Tested
Pig
Predicted

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins (PubMed:26347403). Binds to poly(A) RNA (PubMed:17099224, PubMed:26347403).

Alternative names

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Rabbit Polyclonal RBPMS antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 31 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Activity summary

RBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.

Biological function summary

RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.

Pathways

RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.

Associated diseases and disorders

Mutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.

Product promise

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2 product images

  • Western blot - Anti-RBPMS antibody (ab194213), expandable thumbnail

    Western blot - Anti-RBPMS antibody (ab194213)

    Lanes 1 - 4: Merged signal (red and green). Green - ab194213 observed at 30 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab194213 was shown to react with RBPMS in wild-type HeLa cells in western blot. Here no band was observed in the RBPMS knockout cell line Human RBPMS knockout HeLa cell line ab264697 (RBPMS knockout cell lysate Human RBPMS knockout HeLa cell lysate ab258631), but other antibodies have shown bands in this lysate below 30 kDa that may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and RBPMS knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab194213 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    Lanes 1 - 4: Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-RBPMS antibody (Anti-RBPMS antibody ab152101) at 1/500 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: RBPMS Knockout HeLa cell lysate at 20 µg

    Lane 3: RT-4 cell lysate at 20 µg

    Lane 4: U-251 MG cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 22 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-RBPMS antibody (ab194213), expandable thumbnail

    Western blot - Anti-RBPMS antibody (ab194213)

    All lanes: Western blot - Anti-RBPMS antibody (ab194213) at 1/1000 dilution

    All lanes: Rat heart lysate

    Predicted band size: 22 kDa

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Product protocols

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