AB313829

Anti-RBPMS antibody [HL1105] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal RBPMS antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RBPMS.

View Alternative Names

HERMES, RBPMS, RNA-binding protein with multiple splicing, RBP-MS, Heart and RRM expressed sequence, Hermes

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Paraffin-embedded rat eye tissue stained for RBPMS protein at cytoplasm and nucleus with ab313829 at a 1/250 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Paraffin-embedded mouse eye tissue stained for RBPMS protein at cytoplasm and nucleus with ab313829 at a 1/250 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Paraffin-embedded mouse tissue stained for RBPMS protein with ab313829 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling RBPMS using ab313829 at a 1/100 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Supplier Data

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/10000 dilution

Lane 1:

Non-transfected (–) HCT116 whole cell extracts at 60 µg

Lane 2:

Transfected (+) HCT116 whole cell extracts at 60 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

All lanes:

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell extract at 30 µg

Lane 2:

NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/1000 dilution

Lane 1:

Mouse heart tissue extract at 50 µg

Lane 2:

Rat heart tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1105

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RBPMS.

Q93062

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.

Biological function summary

RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.

Pathways

RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.

Mutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Isoform A. RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS) (PubMed : 24860013, PubMed : 26347403). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets (By similarity). Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state (By similarity). Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats (PubMed : 24860013, PubMed : 26347403). Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes (By similarity).. Isoform C. Acts as a regulator of pre-mRNA alternative splicing (AS) (By similarity). Binds mRNA (PubMed : 17099224). Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA (By similarity). Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins (PubMed : 17099224).

See full target information RBPMS

Additional targets

Rbpms

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBPMS antibody [HL1105] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise