Skip to main content

Rabbit Recombinant Monoclonal RBPMS antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RBPMS.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829), expandable thumbnail
  • Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829), expandable thumbnail
  • Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (AB313829), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human RBPMS. Database link Q93062

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Mouse
Dilution info
1/500 - 1/3000
Notes

-

Species
Rat
Dilution info
1/500 - 1/3000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Isoform A. RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS) (PubMed:24860013, PubMed:26347403). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets (By similarity). Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state (By similarity). Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats (PubMed:24860013, PubMed:26347403). Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes (By similarity). Isoform C. Acts as a regulator of pre-mRNA alternative splicing (AS) (By similarity). Binds mRNA (PubMed:17099224). Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA (By similarity). Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins (PubMed:17099224).

Additional Targets

Rbpms

Alternative names

HERMES, RBPMS, RNA-binding protein with multiple splicing, RBP-MS, Heart and RRM expressed sequence, Hermes

Recommended products

Rabbit Recombinant Monoclonal RBPMS antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human RBPMS.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human RBPMS. Database link Q93062
Carrier free
Yes
Clone number
HL1105
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RBPMS also known as RNA Binding Protein with Multiple Splicing is an important component in RNA processing. It weighs approximately 24 kDa and is widely expressed in human tissues with significant presence in the central nervous system and heart. The protein engages directly with RNA molecules impacting their stability and translation. Its structure allows it to bind specifically to RNA influencing the fate of transcripts by modulating their processing and transport.

Biological function summary

RBPMS plays a role in the regulation of gene expression by interacting with other proteins to form ribonucleoprotein complexes. These complexes are important for the post-transcriptional management of gene expression impacting mRNA splicing and localization. RBPMS also exhibits involvement in the development of neuronal cells where it contributes to neuronal differentiation and growth by aiding in the precise processing of specific mRNA transcripts that are essential for neural functions.

Pathways

RBPMS participates in the regulation of the mRNA splicing and transport pathways. It interacts with the spliceosome complex influencing alternative splicing events that generate diverse protein isoforms. RBPMS is associated with proteins such as SMN1 which also plays a part in mRNA processing and is integral to the spliceosomal machinery. These interactions make RBPMS a pivotal mediator in translating genetic information into functional proteins therefore affecting cellular growth and differentiation.

Associated diseases and disorders

Mutations or altered expression of RBPMS have links to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and cardiovascular disorders. In ALS RBPMS along with proteins like TDP-43 becomes misregulated contributing to disease pathogenesis by disrupting normal RNA processing and splicing patterns. In the heart compromised expression levels of RBPMS can lead to aberrant cardiac function where it might associate with proteins such as Troponin T implicating it in heart muscle contraction regulation essential for maintaining cardiac health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Paraffin-embedded mouse tissue stained for RBPMS protein with ab313829 at a 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Paraffin-embedded mouse eye tissue stained for RBPMS protein at cytoplasm and nucleus with ab313829 at a 1/250 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Paraffin-embedded rat eye tissue stained for RBPMS protein at cytoplasm and nucleus with ab313829 at a 1/250 dilution in immunohistochemical analysis.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Samples were seperated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/10000 dilution

    Lane 1: Non-transfected (–) HCT116 whole cell extracts at 60 µg

    Lane 2: Transfected (+) HCT116 whole cell extracts at 60 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 21 kDa

  • Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Samples were seperated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/1000 dilution

    Lane 1: Mouse heart tissue extract at 50 µg

    Lane 2: Rat heart tissue extracts at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 21 kDa

  • Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    All lanes: Western blot - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma cell line) whole cell extract at 30 µg

    Lane 2: NCI-H1299 (Human lung carcinoma cell line) whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBPMS antibody [HL1105] - BSA and Azide free (ab313829)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue labeling RBPMS using ab313829 at a 1/100 dilution.
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com