AB181098

Anti-RBPMS2 antibody [EPR13121-79]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal RBPMS2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

RNA-binding protein with multiple splicing 2, RBPMS2

6 Images

Lab

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (ab181098)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution

Lane 1:

PC3 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution

All lanes:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 22 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/2000 dilution

Lane 1:

PC-3 lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa,26 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (ab181098) at 1/10000 dilution

All lanes:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa,26 kDa

false

Carrier free

Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13121-79

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RNA-binding protein with multiple splicing 2 commonly known as RBPMS2 or HERMES plays an important mechanical role as a regulator of RNA processing. With a molecular mass of approximately 25 kDa RBPMS2 acts in various tissues including heart eye and smooth muscle cells. Its expression is often specific and changes dynamically during development and in response to different cellular signals hinting at a control function in tissue-specific gene expression.

Biological function summary

By binding to RNA RBPMS2 influences mRNA stability and splicing directly affecting gene expression levels in cells. RBPMS2 is known to be involved in the formation of complexes with other proteins that regulate RNA splicing and transport. Its involvement in these complexes emphasizes its contribution to maintaining proper cellular function and development which is critical in the context of muscle tissue and eye development.

Pathways

RBPMS2 participates in essential signaling pathways such as Wnt signaling and TGF-beta signaling where it interacts with components vital for transcriptional regulation and cytoskeletal organization. This protein's interactions with factors like SMADs in the TGF-beta pathway and beta-catenin in the Wnt pathway help modulate key events in cell proliferation and differentiation illustrating its integrative role in cellular signaling networks.

Studies suggest that RBPMS2 is linked to conditions such as cataracts and cardiomyopathies. Mutations or dysregulation in RBPMS2 expression can disrupt normal cellular activities contributing to disease progression. Protein interactions such as those with SMAD family are significant in understanding its role in abnormal tissue development and associated pathologies offering potential insights into therapeutic intervention strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

RNA-binding protein involved in the regulation of smooth muscle cell differentiation and proliferation in the gastrointestinal system (PubMed : 25064856). Binds NOG mRNA, the major inhibitor of the bone morphogenetic protein (BMP) pathway. Mediates an increase of NOG mRNA levels, thereby contributing to the negative regulation of BMP signaling pathway and promoting reversible dedifferentiation and proliferation of smooth muscle cells (By similarity). Acts as a pre-mRNA alternative splicing regulator (By similarity). Mediates ACTN1 and FLNB alternative splicing (By similarity). Likely binds to mRNA tandem CAC trinucleotide or CA dinucleotide motifs (PubMed : 24860013).

See full target information RBPMS2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 19:1596464 PubMed40463588

2025

Differential retinal ganglion cell resilience to optic nerve injury across vertebrate species.

Applications

Unspecified application

Species

Unspecified reactive species

Julie D De Schutter,Anyi Zhang,Pieter-Jan Serneels,Lieve Moons,Luca Masin,Steven Bergmans

Cell reports 35:109198 PubMed34077720

2021

Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shlomi Dvir,Amir Argoetti,Chen Lesnik,Mark Roytblat,Kohava Shriki,Michal Amit,Tamar Hashimshony,Yael Mandel-Gutfreund

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBPMS2 antibody [EPR13121-79]

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (AB181098)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise