AB250345

Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RBPMS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RNA-binding protein with multiple splicing 2, RBPMS2

6 Images

Lab

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>)

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>) at 1/10000 dilution

Lane 1:

PC3 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

false

Supplier Data

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>) at 1/10000 dilution

All lanes:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 22 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>) at 1/2000 dilution

Lane 1:

PC-3 lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa,26 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>) at 1/10000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa

false

Lab

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

This data was developed using ab181098, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RBPMS2 antibody [EPR13121-79] (<a href='/en-us/products/primary-antibodies/rbpms2-antibody-epr13121-79-ab181098'>ab181098</a>) at 1/10000 dilution

All lanes:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 24 kDa,26 kDa

false

Unconjugated

Anti-RBPMS2 antibody [EPR13121-79]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13121-79

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250345 is the carrier-free version of ab181098.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RNA-binding protein with multiple splicing 2 commonly known as RBPMS2 or HERMES plays an important mechanical role as a regulator of RNA processing. With a molecular mass of approximately 25 kDa RBPMS2 acts in various tissues including heart eye and smooth muscle cells. Its expression is often specific and changes dynamically during development and in response to different cellular signals hinting at a control function in tissue-specific gene expression.

Biological function summary

By binding to RNA RBPMS2 influences mRNA stability and splicing directly affecting gene expression levels in cells. RBPMS2 is known to be involved in the formation of complexes with other proteins that regulate RNA splicing and transport. Its involvement in these complexes emphasizes its contribution to maintaining proper cellular function and development which is critical in the context of muscle tissue and eye development.

Pathways

RBPMS2 participates in essential signaling pathways such as Wnt signaling and TGF-beta signaling where it interacts with components vital for transcriptional regulation and cytoskeletal organization. This protein's interactions with factors like SMADs in the TGF-beta pathway and beta-catenin in the Wnt pathway help modulate key events in cell proliferation and differentiation illustrating its integrative role in cellular signaling networks.

Studies suggest that RBPMS2 is linked to conditions such as cataracts and cardiomyopathies. Mutations or dysregulation in RBPMS2 expression can disrupt normal cellular activities contributing to disease progression. Protein interactions such as those with SMAD family are significant in understanding its role in abnormal tissue development and associated pathologies offering potential insights into therapeutic intervention strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein involved in the regulation of smooth muscle cell differentiation and proliferation in the gastrointestinal system (PubMed : 25064856). Binds NOG mRNA, the major inhibitor of the bone morphogenetic protein (BMP) pathway. Mediates an increase of NOG mRNA levels, thereby contributing to the negative regulation of BMP signaling pathway and promoting reversible dedifferentiation and proliferation of smooth muscle cells (By similarity). Acts as a pre-mRNA alternative splicing regulator (By similarity). Mediates ACTN1 and FLNB alternative splicing (By similarity). Likely binds to mRNA tandem CAC trinucleotide or CA dinucleotide motifs (PubMed : 24860013).

See full target information RBPMS2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free

Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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