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Rabbit Recombinant Monoclonal RBPMS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345), expandable thumbnail
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345), expandable thumbnail
  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345), expandable thumbnail
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345), expandable thumbnail
  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (AB250345), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

RNA-binding protein involved in the regulation of smooth muscle cell differentiation and proliferation in the gastrointestinal system (PubMed:25064856). Binds NOG mRNA, the major inhibitor of the bone morphogenetic protein (BMP) pathway. Mediates an increase of NOG mRNA levels, thereby contributing to the negative regulation of BMP signaling pathway and promoting reversible dedifferentiation and proliferation of smooth muscle cells (By similarity). Acts as a pre-mRNA alternative splicing regulator (By similarity). Mediates ACTN1 and FLNB alternative splicing (By similarity). Likely binds to mRNA tandem CAC trinucleotide or CA dinucleotide motifs (PubMed:24860013).

Alternative names

RNA-binding protein with multiple splicing 2, RBPMS2

Recommended products

Rabbit Recombinant Monoclonal RBPMS2 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13121-79
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250345 is the carrier-free version of Anti-RBPMS2 antibody [EPR13121-79] ab181098.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The RNA-binding protein with multiple splicing 2 commonly known as RBPMS2 or HERMES plays an important mechanical role as a regulator of RNA processing. With a molecular mass of approximately 25 kDa RBPMS2 acts in various tissues including heart eye and smooth muscle cells. Its expression is often specific and changes dynamically during development and in response to different cellular signals hinting at a control function in tissue-specific gene expression.

Biological function summary

By binding to RNA RBPMS2 influences mRNA stability and splicing directly affecting gene expression levels in cells. RBPMS2 is known to be involved in the formation of complexes with other proteins that regulate RNA splicing and transport. Its involvement in these complexes emphasizes its contribution to maintaining proper cellular function and development which is critical in the context of muscle tissue and eye development.

Pathways

RBPMS2 participates in essential signaling pathways such as Wnt signaling and TGF-beta signaling where it interacts with components vital for transcriptional regulation and cytoskeletal organization. This protein's interactions with factors like SMADs in the TGF-beta pathway and beta-catenin in the Wnt pathway help modulate key events in cell proliferation and differentiation illustrating its integrative role in cellular signaling networks.

Associated diseases and disorders

Studies suggest that RBPMS2 is linked to conditions such as cataracts and cardiomyopathies. Mutations or dysregulation in RBPMS2 expression can disrupt normal cellular activities contributing to disease progression. Protein interactions such as those with SMAD family are significant in understanding its role in abnormal tissue development and associated pathologies offering potential insights into therapeutic intervention strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098) at 1/2000 dilution

    Lane 1: PC-3 lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: Human fetal liver tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 24 kDa, 26 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098) at 1/10000 dilution

    All lanes: Mouse heart tissue lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 24 kDa

  • Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.Anti-RBPMS2 antibody [EPR13121-79] ab181098 (purified) at 1/50 immunoprecipitating RBPMS2 in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098)

    Predicted band size: 22 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098) at 1/10000 dilution

    All lanes: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 24 kDa, 26 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098) at 1/10000 dilution

    Lane 1: PC3 cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

  • Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345), expandable thumbnail

    Western blot - Anti-RBPMS2 antibody [EPR13121-79] - BSA and Azide free (ab250345)

    This data was developed using Anti-RBPMS2 antibody [EPR13121-79] ab181098, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RBPMS2 antibody [EPR13121-79] (Anti-RBPMS2 antibody [EPR13121-79] ab181098) at 1/10000 dilution

    All lanes: Human fetal liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 22 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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