Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RBX1 with Purified ab133565 at 1 : 50 dilution (3.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling RBX1 with Purified ab133565 at 1 : 20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling RBX1 with Purified ab133565 at 1 : 2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IP

Supplier Data

Immunoprecipitation - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.

Purified ab133565 at 1 : 20 dilution (1μg) immunoprecipitating RBX1 in Jurkat whole cell lysate.

Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg.

Lane 2 (+) : ab133565 + Jurkat whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab133565 in Jurkat whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1 : 1000 dilution) was used for Western blotting.

Blocking Buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM/TBST.

Observed band size : 13 kDa

All lanes:

Immunoprecipitation - Anti-RBX1 antibody [EPR6850(B)] (<a href='/en-us/products/primary-antibodies/rbx1-antibody-epr6850b-ab133565'>ab133565</a>)

Predicted band size: 12 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat breast tissue sections labeling RBX1 with Purified ab133565 at 1 : 2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling RBX1 with Purified ab133565 at 1 : 2000 dilution (0.099 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (AB248556)

This data was developed using ab133565, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Image produced using the purified version.

All lanes:

Western blot - Anti-RBX1 antibody [EPR6850(B)] (<a href='/en-us/products/primary-antibodies/rbx1-antibody-epr6850b-ab133565'>ab133565</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

Mouse heart lysate at 15 µg

Lane 3:

Rat heart lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 13 kDa

false