AB70195

Anti-RC3H1 antibody

Name: Anti-RC3H1 antibody (ab70195) | Abcam
Brand: Abcam Limited
SKU: AB70195
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RC3H1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RC3H1.

View Alternative Names

KIAA2025, RNF198, RC3H1, Roquin-1, Roquin, RING finger and C3H zinc finger protein 1, RING finger and CCCH-type zinc finger domain-containing protein 1, RING finger protein 198

3 Images

Supplier Data

Immunoprecipitation - Anti-RC3H1 antibody (AB70195)

RC3H1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70195 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab70195 at 0.1 μg/mL.

Lane 1 : ab70195 IP in HeLa whole cell lysate.

Lane 2 : ab70195 IP in HeLa whole cell lysate.

Lane 3 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-RC3H1 antibody (ab70195)

Predicted band size: 126 kDa

false

Supplier Data

Western blot - Anti-RC3H1 antibody (AB70195)

All lanes:

Western blot - Anti-RC3H1 antibody (ab70195) at 2 µg/mL

Lane 1:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 2:

4T1 (Mouse mammary gland carcinoma cell line) whole cell lysate at 50 µg

Lane 3:

CT26.WT (Murine Colon Carcinoma) whole cell lysate at 50 µg

Predicted band size: 126 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-RC3H1 antibody (AB70195)

All lanes:

Western blot - Anti-RC3H1 antibody (ab70195) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Predicted band size: 126 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RC3H1. The exact immunogen used to generate this antibody is proprietary information.

Q5TC82

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70195 was affinity purified using an epitope specific to RC3H1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RC3H1 also known as Roquin-1 is a protein with a mass of approximately 125 kDa. It plays a mechanical role in regulating mRNA stability and modulating the immune response by binding to specific mRNA transcripts. RC3H1 contains a unique RING finger and CCCH-type zinc finger domains that assist in recognizing and binding to target RNAs. Expression of RC3H1 occurs in various tissues including lymphoid organs indicating its involvement in immune system regulation.

Biological function summary

RC3H1 contributes to the control of immune homeostasis and inflammation. It acts as part of a complex with other signaling molecules regulating the degradation of target mRNAs by interacting with decay-inducing deadenylation molecules. Its activity ensures the proper regulation of immune responses preventing autoimmunity and excessive inflammation. The protein exerts its effects by targeting mRNAs of cytokines and other immune-related proteins.

Pathways

RC3H1 connects to key immune regulation and inflammatory pathways such as the NF-κB signaling and T-cell receptor signaling pathways. Through these pathways RC3H1 influences the stability and expression of proteins like TNF-alpha and ICOS which are essential for immune responses. These interactions help maintain a balance in immune cell activation and proliferation impacting overall immune function.

RC3H1 has been linked to autoimmunity and chronic inflammatory conditions. Its connection to the development of these diseases involves dysregulation of mRNA degradation leading to alterations in cytokine production and immune cell activation. For instance compromised RC3H1 function associates with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) where imbalanced immune responses occur. Studies suggest that RC3H1's interaction with related proteins such as CTLA-4 and other co-stimulatory molecules contributes to the pathological manifestations of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs (PubMed : 25026078, PubMed : 31636267). Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-independent manner (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity). Recognizes and binds mRNAs containing a hexaloop stem-loop motif, called alternative decay element (ADE) (By similarity). Together with ZC3H12A, destabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR (By similarity). Able to interact with double-stranded RNA (dsRNA) (PubMed : 25026078, PubMed : 25504471). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed : 25697406, PubMed : 31636267). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2A, UBE2B, UBE2D2, UBE2F, UBE2G1, UBE2G2 and UBE2L3 and produces polyubiquitin chains (PubMed : 26489670). Shows the strongest activity when paired with UBE2N : UBE2V1 or UBE2N : UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed : 26489670).

See full target information RC3H1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 26:382-393 PubMed30283082

2018

Hypoxia-induced autophagy of stellate cells inhibits expression and secretion of lumican into microenvironment of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xinqun Li,Yeonju Lee,Ya'an Kang,Bingbing Dai,Mayrim Rios Perez,Michael Pratt,Eugene J Koay,Michael Kim,Rolf A Brekken,Jason B Fleming

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RC3H1 antibody

Immunoprecipitation - Anti-RC3H1 antibody (AB70195)

Western blot - Anti-RC3H1 antibody (AB70195)

Western blot - Anti-RC3H1 antibody (AB70195)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise