AB251310

Anti-RCAS1 antibody [EPR16568] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RCAS1 antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RCAS1, EBAG9, Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HEK293 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200348 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized T-47D (Human ductal breast epithelial tumor cell line) cells, labeling RCAS1 with ab200348 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on T47D cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

Supplier Data

Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

RCAS1 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200348 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab200348 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HEK293 whole cell lysate 10 μg (Input). Lane 2 : ab200348 IP in HEK293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200348 in HEK293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] (<a href='/en-us/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>)

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/en-us/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/1000 dilution

All lanes:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 32 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/en-us/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood ) whole cell lysate at 10 µg

Lane 2:

293 (Human embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa,32 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

This data was developed using ab200348, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RCAS1 antibody [EPR16568] (<a href='/en-us/products/primary-antibodies/rcas1-antibody-epr16568-ab200348'>ab200348</a>) at 1/5000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16568

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab251310 is the carrier-free version of ab200348.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RCAS1 also known as Receptor-binding Cancer Antigen expressed on SiSo cells or Estrogen Receptor Binding Fragment Associated Gene 9 (EBAG9) is a membrane-bound protein with an approximate mass of 32 kDa. It is widely expressed in various tissues including the reproductive system lungs and liver. This protein acts mechanically as a ligand for the RCAS1 receptor facilitating cell-to-cell communication through binding.

Biological function summary

RCAS1 plays a significant role in immune modulation by influencing cell death and immune response. Researchers have not classified it as being a part of any known protein complex. This protein helps regulate the immune system's ability to recognize and respond to abnormal or cancerous cells. It contributes to creating an immune-suppressive microenvironment favoring tumor growth by inhibiting the function of natural killer cells and cytotoxic T lymphocytes.

Pathways

RCAS1's functions intersect with important processes in cellular immune regulation and apoptosis. One of the major pathways involves the Fas signaling pathway where RCAS1 interacts with Fas-related proteins affecting apoptotic cell death. Through this pathway it modulates immune escape mechanisms employed by tumor cells. RCAS1 also correlates with pathways linked to inflammatory responses including its interaction with cytokines such as interferon-gamma.

RCAS1 has significant implications in oncology particularly in various cancers like breast and cervical cancer. Its expression level often shows correlation with tumor aggressiveness and poor prognosis. RCAS1's expression may engage other proteins such as progesterone receptors impacting hormone-related pathways in breast cancer. Additionally the protein's role in immune suppression indicates its potential involvement in immune escape by tumors which makes it a focus of study in cancer immunotherapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

See full target information EBAG9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 16: PubMed38732558

2024

Differential Modulation by Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) of Mesenteric Fat and Macrophages and T Cells in Adipose Tissue of Obese / Zucker Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Hong,Peter Zahradka,Carla G Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RCAS1 antibody [EPR16568] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Immunocytochemistry/ Immunofluorescence - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Immunoprecipitation - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Western blot - Anti-RCAS1 antibody [EPR16568] - BSA and Azide free (AB251310)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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